摘要
目的:研究皮肤恶性黑素瘤(MM)组织中血管内皮生长因子C(vascular endothelial growth factor-C,VEGF-C)及其受体VEGFR-3(flt-4)的表达情况,分析其在肿瘤淋巴管的生长及淋巴转移中的作用。方法:采用免疫组化SP法分析29例MM石蜡标本中VEGF-C/flt-4蛋白的表达情况,并与20例色素痣石蜡标本中VEGF-C/flt-4蛋白表达情况相对比,统计分析其与临床病理特点之间的关系。结果:VEGF-C蛋白的阳性率为96.6%(28/29),flt-4的阳性率为86.2%(25/29),明显高于色素痣(P<0.01),与肿瘤的病理分级、临床分期和肿瘤的淋巴转移显著相关(P<0.05);VEGF-C和flt-4的阳性表达相一致。结论:VEGF-C/flt-4在皮肤恶性黑素瘤中的表达明显高于色素痣,与肿瘤的预后密切相关,在肿瘤淋巴转移中可能有重要作用。
Objective： To determine the expression of vascular endothelial growth factor- C and its receptor in malignant melanoma （MM） and to elucidate their role in lymphonode metastasis and clinical significance. Methods： Tissue sections from 29 MM patients were examined. The expression of VEGF - C and its receptor were detected us- ing SP immunohistochemistry technique and the relationship between the VEGF- C/VEGFR- 3 expression and clinical pathological characteristics was analyzed. Results： VEGF - C and its receptor were determined in 29 cases, which was higher than that in 20 cases of nevus pigmentosus tissue （P 〈 0.01）. Out of 29 cases, VEGF- C and VEGFR- 3 protein expressions were detected in 28 （96.6%） and 25 （86.2%） respectively. There was statistical relationship between VEGF- C and VEGFR- 3. The expression of VEGF- C was correlated with the pathological grade of tumor, clinical stage of the tumor and lymphonode metastasis （ P 〈 0.05）. Conclusion： The results suggest that VEGF- C/VEGFR- 3 may play an important role in the lymphonode metastasis and it can be a useful biological marker to foresee lymphonode metastasis and prognosis.
出处
《中国麻风皮肤病杂志》
2007年第5期379-381,共3页
China Journal of Leprosy and Skin Diseases
关键词
恶性黑素瘤
血管内皮生长因子C
血管内皮生长因子受体-3
淋巴转移
malignant melanoma
vascular endothelial growth factor- C
vascular endothelial growth factor receptor - 3
lymphonode matastasis
