摘要
目的探讨胆汁返流与幽门区局部神经递质一氧化氮的变化对应激性溃疡发病的影响及可能的机理。方法采用冷束缚应激方法复制应激性溃疡的动物模型,将60只成年SD大鼠分为两部分,第1部分:对照组10只,溃疡组30只,用于观测影响应激性溃疡发生的相关因素;第2部分:左旋硝基精氨酸甲酯(L-NAME)预处理组10只(L-NAME组),生理盐水预处理组10只(生理盐水组),用于观测幽门区局部一氧化氮含量变化对其他因素的影响。分别检测各组胃内胆汁酸浓度、胃黏膜溃疡指数(Guth评分)以及胃内pH值,用生化试剂盒检测幽门区一氧化氮的含量。结果溃疡组应激结束后1h幽门区一氧化氮含量达到峰值(P<0.01),应激结束后2h胃内胆汁酸浓度和pH值均达到峰值(P<0.01),应激结束后4h胃内溃疡指数达到峰值(P<0.01)。L-NAME组的胃内胆汁酸浓度、溃疡指数及一氧化氮含量较生理盐水组显著降低(P<0.01,P<0.05),而胃内pH值的差异无统计学意义(P>0.05)。幽门区一氧化氮含量与胆汁酸浓度呈正相关(r=0.715,F=12.55,P=0.017)。结论幽门区一氧化氮含量变化与胆汁的返流量之间存在正相关关系,而L-NAME的幽门区局部干预使幽门区一氧化氮含量减少,幽门松弛程度降低,胆汁返流量也随之减少,进而降低溃疡指数,起到保护胃黏膜的作用。
Objective To investigate the effects and possible mechanism of the local content changes of nitrogen monoxidum on bile reflux in rats＇ pylori stress ulcer. Methods Fifty adult SD rats were restrained in planes and then were soaked in cold water to make stress ulcer. They were divided into three groups randomly： the stress group （30 cases）, the L-NAME group （10 cases）, whose pylori were injected with L-NAME solution and the normal saline group （10 cases）, whose pylori were treated with saline solution. Besides, there were another 10 rats taken as the control group. The gastric ulcer index, pH value and bile acid concentration of gastric juice in each group were measured before and after stress. The local concentrations of nitrogen monoxidum in rats＇ pylori were also measured by nitrogen monoxidum Biochemical Detection Kit. Results The local content of nitrogen monoxidum in rats＇ pylori increased to maximum at 1 h after stress （P〈0.01）, while the bile acid and pH value of gastric juice went up to the maximum at 2 h after stress （P〈0.01）. The ulcer index reached the peak value at 4 h after stress （P〈0. 01）. The bile acid concentration and ulcer index of the group injected with L-NAME solution in rats＇ pylori decreased significantly compared with those of the group treated with normal saline solution （P〈0.01）. The local concentration of nitrogen monoxidum also decreased significantly in the L-NAME group （P〈0.05）. However, there was no difference on pH values of gastric juice between those two groups （P〉0.05）. Conclusion There is a positive correlation between the local concentration of nitrogen monoxidum in pylori and bile acid in the stomach in rats with stress ulcer. Injection of L-NAME solution in rats＇ pylori may reduce the local content of nitrogen monoxidum so that the pyloric sphincter become less tense, which may lead to the significant decreases of ulcer index and bile acid in the stomach. Thus, L-NAME may protect gastric mucus effectively.
出处
《中国普外基础与临床杂志》
CAS
2007年第3期300-303,共4页
Chinese Journal of Bases and Clinics In General Surgery
