摘要
目的研究胃正常组织、不典型增生组织和癌组织中Ki67和PTEN蛋白的表达情况,探讨其与胃组织癌变的关系及作为早期癌变生物学标志的可能性。方法应用免疫组织化学SP法对22例胃正常组织、80例不典型增生组织及60例胃癌组织中Ki67和PTEN蛋白的表达情况进行检测。结果在胃正常组织、不典型增生组织及胃癌组织中PTEN蛋白的表达阳性率呈递减趋势,Ki67蛋白则呈递增趋势;等级相关分析结果显示PTEN和Ki67表达阳性率与胃黏膜组织从正常至不典型增生再至癌变这一过程均相关(r分别为-0.461和0.301,P<0.01)。PTEN的表达与胃癌的Lauren分型、分化程度、浸润深度无关,与淋巴结转移有关。Ki67的表达与胃癌的Lauren分型、淋巴结转移有关,与浸润深度、分化程度无关。PTEN和Ki67蛋白的表达在胃癌组织中呈负相关(r=-0.316,P<0.05)。结论Ki67和PTEN蛋白的表达异常与胃癌的癌变过程相关,两者有可能成为胃组织早期癌变的分子标记物。
Objective To investigate the expressions of Ki67 and PTEN protein in human gastric atypical hyperplasia and carcinoma, and to explore their relations with gastric canceration and the possibility of being early biological markers. Methods The expressions Ki67 and PTEN protein were detected by using immunohistochemical staining SP method in 22 cases of normal gastric tissues and 80 cases of atypical hyperplasia and 60 cases of gastric carcinoma. Results The positive expression level of PTEN in normal gastric tissue was higher than that in atypical hyperplasia tissue, with the lowest level in gastric carcinoma, whereas, it was opposite for the expressions of Ki67 in these samples. The positive expression rates of PTEN and Ki67 were both well related with pathological grading of gastric tissues （r= -0. 461 and 0. 301, respectively, P〈0.01）. The expression of PTEN was not obviously related with Lauren type, infiltration degree and differentiation level of gastric carcinoma, but it was associated with lymph node metastasis. For Ki67, it was found that there were correlations between the expression of Ki67 and Lauren type, lymph node metastasis, but not with infiltration degree, differentiation level of gastric carcinoma. Negative correlation was found between the expressions of PTEN and Ki67 protein in gastric carcinoma （r=-0. 316, P〈0. 05）. Conclusion Abnormal expressions of Ki67 and PTEN protein were associated with canceration in stomach. Therefore, both of them may act as useful biomarkers for early detection of gastric carcinoma.
出处
《中国普外基础与临床杂志》
CAS
2007年第3期304-307,共4页
Chinese Journal of Bases and Clinics In General Surgery
基金
中国科学院西部之光计划(项目编号:20000132)~~
