摘要
目的观察急性胰腺炎大鼠血中C肽(CP)、胰岛素(INS)、胰高糖素(GG)和血糖水平,以揭示胰岛内分泌细胞的功能变化。方法将120只大鼠随机平均分为A组(急性胰腺炎)和B组(假手术对照组),每组60只。用放射免疫分析法测定大鼠血中CP,INS和GG水平,同时测定血糖值。大鼠剖腹后肉眼观察胰腺形态学变化,取胰腺标本行组织病理学检查。结果制模9h后病理检查示A组大鼠均表现为重型急性胰腺炎。A组CP于术后9h,INS于术后15h和GG于术后12h开始明显高于对照组(P<0.01,P<0.05,P<0.05)。结论(1)急性胰腺炎时大鼠血中CP和INS水平可反映胰岛B细胞功能受损;CP能更准确、更及时地反映胰岛B细胞受损程度,并与病理学变化同步。(2)GG水平可反映胰岛A细胞功能;急性胰腺炎时A细胞可能比B细胞更易于受到破坏。
Objective To investigate the levels of C peptide, insulin, glucagon and glucose and the function of islet endocrine cells in rats with acute pancreatitis. Methods one hundred and tweenty rats were randomly divided to two groups ： Group A （ acute pancreatitis, AP ） and Group B （ sham operation, SO ）. Blood C peptide, insulin, glucagon and glucose were determined. The pancreatic morphology was observed after laparotomy. Pancreas tissue was excised to be observed in terms of histopathology. Results The levels of C peptide, insulin and glucagon in group A were significantly higher than those in the group B at 9 h, 15 h and 12h after the procedure respectively （P 〈 0. 01, P 〈 0. 05 and P 〈 0. 05, respectively ）. Histopathologjcally, the rats in group A had severe acute pancreatitis at 9h after the procedure. Conclusions （1）The islet endocrine cells function of rats with acute pancreatitis is impaired, the levels of C-peptide and insulin in serum can reflect the function of islet B cells. C-peptide can more accurately and more quickly reflect the degree of B cells injury, and its changes are synchronous with the histopathological changes in B cells. （ 2 ） The level of glucagon can reflect the function of pancreas A cells. The islet A cells may be more susceptible than B cells to be damaged in acute pancreatitis.
出处
《中国普通外科杂志》
CAS
CSCD
2007年第5期426-428,共3页
China Journal of General Surgery
