摘要
目的:研究阿胶有效组分A、B对放射损伤小鼠造血系统的保护作用机制。方法:小鼠3.5Gy137Se全身辐射造模,每日给药A(1.0g/kg和2.0g/kg)和B(0.8g/kg和1.6g/kg),d25处死动物。检测小鼠骨髓和脾造血干/祖细胞集落红系暴增式集落形成单位(BFU-E)、红系集落形成单位(CFU-E)、粒细胞巨噬细胞集落生成单位(CFU-GM)、脾表面结节数量,荧光酶标仪检测骨髓细胞活性氧(ROS)含量,Elisa测定小鼠外周血粒-巨噬细胞集落刺激因子(GM-CSF)、IL-6、红细胞生成素(EPO)、IFN-γ、β型转化生长因子(TGF-β)和血清及肝组织匀浆内超氧化物歧化酶(SOD)和谷胱甘肽过氧物酶(GSH-Px)含量。结果:A、B组分能促进射线损伤小鼠外周血白细胞和红细胞的升高,保护骨髓和脾造血干/祖细胞集落BFU-E、CFU-E、CFU-GM,增加脾表面集落形成单位(CFU-S)数量和血清中GM-CSF、IL-6含量,降低骨髓细胞内ROS含量,提高血清内SOD、GSH-Px和肝脏内SOD含量。结论:从体外模拟人胃、肠的消化系统能够分离阿胶有效补血活性成分,这种成分保护辐射损伤小鼠造血系统的机制可能与保护贫血小鼠造血微环境、刺激机体表达相关造血细胞因子和增强机体抗自由基能力有关。
AIM： To filrther understand the hematopoietic activities and mechanisms of fraction A （ 1.0 g/kg and 2.0 g/kg） and B （0.8 g/kg and 1.6 g/ks） from enzyme-digested colla corn aslm on the anemic mice caused by 3.5 Gy γ-irradiation. METHODS： The fraction A and B were orally administrated to the anemic mice for 25 days. After confirming the protective effects of fraction A and B, we examined the effects of fraction A and B on CFU-GM, BFU-E, CFU-E in the bone marrow and spleen, cytokines in the serum, CUF-S in the spleen, ROS in bone marrow cells, SOD and GSH-Px in serum and liver homogenate. RESISTS： Fraction A administrated at 2.0 g/kg and 1.0 g/kg and fraction B administrated at 1.6 g/kg and 0.8 g/kg activated granulocyte and erythrocyte progenitor cells in bone marrow and spleen, led to the recovery of white blood cell and red blood cell count to a value that is almost approaching the basal level, increased the colony number of CFU-GM, BFU-E, CFU-E in bone marrow and spleen, stimulated the CFU-S in the spleen, significantly stimulated the secretion of IL-6 and GM-CSF, decreased the ROS level in bone marrow cells, and increased the SOD activity in bone marrow and liver and GSH-Px activity in serum. CONCLUSION： These results suggest that the fraction A and B from the enzyme-digested colla corri asini promote hematopoiesis by activating immature granulocyte and erythrocyte cells, in part by stimulating the GM-CSF and IL-6 secretion and elevating the ROS scavenging ability.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2007年第4期417-421,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
山东东阿阿胶股份有限公司基金项目
国家自然科学基金项目(20572022)
关键词
阿胶
酶解
辐射损伤
造血
保护作用
colla corri asini
enzyme digestion
irradiation
hematopoiesis
