摘要
以7份高粱自交系幼胚为外植体,研究了不同基因型对组织培养的反应和植株再生的差异。结果表明:不同高粱基因型间愈伤组织诱导率没有明显差异,但胚性愈伤组织百分率、不定芽分化率以及再生频率有明显差异。其中YN336A、YN267R、YN510R和YN338A的再生效果较好,其不定芽发生率、不定根发生率及移栽成活率分别为64.4%-72.8%,76.2%-100.0%和36.3%-80.0%。对只生根不生芽的基因型,加入1-2 mg/L KT有明显抑制生根、促进生芽的效应。
Explants of immature embryos of seven genotypes of sorghum inbred lines were examined for their response in tissue culture and plant regeneration. The results indicated that there were no significant differences of induction frequency among different genotypes, but the frequency of regenerable callus formation, shoot regeneration and plant regeneration was significantly different. YN336A, YN267R, YN510R and YN338A showed good performances under regeneration culture conditions, frequency of shoot formation, root formation and transplant survive was in the range of 64.4% -72.8 %, 76.2% - 100.0% and 36.3 % - 80.0 %, respectively. For the genotypes that rooted rather than shot, 1 - 2 mg/L KT could effectively inhibit rooting and promote shooting.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2007年第3期256-258,共3页
Journal of Jilin Agricultural University
基金
国家自然科学基金资助项目(30430060)
关键词
高粱
自交系
幼胚
组织培养
植株再生
sorghum
inbred line
immature embryo
tissue culture
plant regeneration
