摘要
目的探讨考试应激时健康机体血常规的变化及与人类白细胞抗原-DQB(HLA-DQB1)等位基因的关系。方法随机选取51名大学二年级学生,评定应考准备期的心理状况;分别测量学生在应考准备期和正常学习期的血常规指标;HLA-DQB1*02,*03,*04,*05和*06分别利用聚合酶链式反应(PCR)由第二外显子组特异性引物扩增得到,并研究其与血常规各指标间的相关性。结果(1)在应考准备期对学生的健康自评量表(SCL-90)评定中,躯体化、强迫症状、人际关系、焦虑、敌对、偏执、精神病性的得分及阳性数目平均分均显著高于全国青年常模(P<0.05)。(2)应考准备期与正常学习期比较。血常规指标中:白细胞(WBC)、中性粒细胞绝对值(NEUT#)、中性粒细胞百分比(NEUT%)、淋巴细胞绝对值(LYMPH#)、红细胞(RBC)、血红蛋白(HGB)、红细胞压积(HCT)升高,而平均红细胞血红蛋白量(MCH)、平均红细胞血红蛋白浓度(MCHC)均降低,差异有统计学意义(P<0.05)。(3)HLA-DQB1*02与应考准备期的NEUT%呈负相关(P<0.05),与正常学习期的血小板分布宽度(PDW)呈负相关(P<0.05);HLA-DQB1*03与应考准备期的WBC呈负相关(P<0.05);HLA-DQB1*04与应考准备期的RBC、HGB、HCT呈正相关(P<0.01);和红细胞分布宽度(RDW-CV)呈正相关(P<0.05);HLA-DQB1*05与应考准备期的WBC、嗜酸细胞绝对值(EO#)呈正相关(P<0.05);与NEUT#呈负相关(P<0.05);HLA-DQB1*06与应考准备期的血小板(PLT)呈正相关(P<0.05),与正常学习期的PLT、大血小板比率(P-LCR)呈正相关(P<0.05)。结论考试应激可以引起血常规中某些指标显著改变并与HLA-DQB1等位基因表型有关。血常规可以作为观察应激的敏感指标,HLA-DQB1*02,*03,*04为应激基因。
Objective To explore the influences of the exam stress on hematological parameters and their correlations with the phenotype of HLA-DQB1 on healthy volunteers. Methods 51 students of grade two in university were selected randomly and investigate. The hematological parameters during exam preparation period and normal study period were measured respectively. HLA-DQB1＊ 02, ＊03, ＊ 04, ＊ 05 and ＊06 alleles were individually amplified by polymerase chain reaction（PCR） using exon 2 group-specific primers and the correlation with hematological parameters were investigated. Results （ 1 ） Somatization, obsessive-compulsive, interpersonal relationship, anxiety, hostility, paranoid ideation, psychosis score and the number of positive items of SCL-90 in students during exam preparation period were significantly higher than normal （P 〈 0.05 ）. （2） Exam stress significantly increased with the increase of white blood cell （WBC）, absolute value of neutrophil（ NEUT% ）, percentare of neutrophil （ NEUT % ）, absolute value of lymphocyte （ LYMPH % ）, red blood cell （RBC）, hemoglobin （ HGB ）, haernatocrit （HCT）, while decreased mean corpuscular hemoglobin （MCH）, mean corpuscular hemoglobin concentration （MCHC）. （3）HLA-DQB1＊ 02 was negative correlated with NEUT% during exam preparation period and with platelet distribution width （PDW） during normal study period. HLA-DQB1 ＊03 was negative correlation with WBC during exam preparation period. HLA-DQB1＊04 was positive correlated with RBC, HGB, HCT and red cell distribution width-corpuscular volume （RDW-CV） during exam preparation period. HLA-DQBI＊05 was positive correlated with WBC, aboslute value of eosinophil（EO #） during exam preparation period and negative correlation with NEUT # during normal study period. HLA- DQB1＊ 06 was positive correlated with platelet （PLT） during exam preparation period, and with PLT, percentage of large platelet （P-LCR） during normal study period. Conclusion Exam stress induces significant hematological changes and there are correlations with the alleles of HLA-DQB1. These changes are related to the measures of perceived stress. HLA-DQB1＊02, ＊03, ＊04 are stress related gene.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第6期717-719,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(30370484)
