摘要
目的:探讨中药联合胸段硬膜外阻滞对哮喘大鼠肺泡灌洗液GM-CSF、血清中IL-12水平的影响。方法:将健康大鼠40只(雌雄各半)按随机数字表将其分为5组:空白组(A组)、哮喘模型组(B组)、中药组(C组)、胸段硬膜外阻滞组(D组)、胸段硬膜外阻滞+中药组(E组)。建立大鼠哮喘模型以及胸段硬膜外阻滞的模型,采用中药灌胃及硬膜外给药,用ELISA法检测大鼠血清当中IL-12、肺泡灌洗液中GM-CSF水平的变化。结果:哮喘大鼠血清中IL-12水平明显降低,肺泡灌洗液中GM-CSF水平明显升高。中药与硬膜外阻滞以及二者的联合应用均可抑制IL-12水平的下降和GM-CSF水平的升高。二者联合应用组IL-12的下降、GM-CSF水平的升高低于中药组与单纯硬膜外阻滞组。中药组与单纯硬膜外阻滞组IL-12、GM-CSF含量无显著性差异。结论:胸段硬膜外阻滞联合中药对IL-12的下降及GM-CSF的升高有明显的抑制作用。
Objective： To observe the changes of GM - CSF concentration in bronchoalveolar lavage fluid（BALF） and IL - 12 concentration in serum of asthmatic rat after the rats were given the thoracic epidural blook combined with Chinese herb. Methods. 40 cases of healthy SD rat（20 male and 20 female） were randomized divided into five groups, i.e. blank group（ A group）, asthma group （B group）, Chinese herb （C group）, epidural block （D group）, Chinese herb combined with epidural block （E group）. B,C, D and E group were used to make asthmatic model. D and E group were used to make epidural block model homogenously. Chinese herb were given to C and E group, Bupivicaine was given from the path of epidural block to D and E group. ELISA method was applied to detect IL - 12 concentration of the serum and GM - CSF concentration of the BALF. Results： B group compared with A group, IL - 12 concentration of serum showed lower obviously and GM - CSF concentration of the BALF showed higher obviously. The C and D group compared with the B group, decreasing the concentration of the BALF and increasing the concentration of the serum. There was no significant difference between the E group and the A group. Conclusion： Chinese herb combined with epidural block can constrict the decreasing of the IL - 12 concentration in the serum and the increasing of the GM - CSF concentration in the BALF.
出处
《中华中医药学刊》
CAS
2007年第6期1101-1103,共3页
Chinese Archives of Traditional Chinese Medicine
基金
黑龙江省自然科学基金资助项目(D2004-31)
