摘要
目的探讨丹皮酚单药及联合顺铂应用对人食管癌Eca-109细胞的增殖抑制作用。方法以不同浓度的丹皮酚、顺铂和两药联用处理人食管癌Eca-109细胞,采用MTT法测定药物对体外培养的人食管癌Eca-109细胞增殖的抑制作用,应用两药相互作用指数(coefficient of drug interaction,CDI)进行联合用药分析。结果丹皮酚(7.81～250 mg.L-1)和顺铂(0.078～5 mg.L-1)单独应用均对人食管癌Eca-109细胞有抑制作用,呈现剂量效应关系。丹皮酚与顺铂联合应用时,丹皮酚能明显提高顺铂对Eca-109细胞的增殖抑制作用,有显著的协同杀伤作用。结论丹皮酚与顺铂联合应用具有明显的协同抗食管癌Eca-109细胞增殖的作用。
Aim To investigate the inhibitory effect of paeonol and it＇s combination with cisplatin on the proliferation of human esophageal cancer cell line Eca-109. Methods The inhibitory effect of drugs with different concentration on the proliferation of human esophageal cancer cell line Eca-109 in virto was measured by MTr assay. The coefficient of drug interaction（CDI） was used to analyse the synergistically inhibitory effect of drug combination. Results Paeonol （7.81 - 250 mg·L^- 1 ） and cisplatin （0. 078 -5 mg·L^- 1 ） had inhibitory effects on the proliferation of human esophageal cancer cell line Eca-109 with dose-dependent manner. Paeonol and cisplatin had synergetic effect in inhibiting the proliferation of Eca-109 cells. Conclusion The results suggest that the combination of paeonol and cisplatin has synergistically inhibitory effect on the proliferation of human esophageal cancer cell line Eca-109.
出处
《安徽医药》
CAS
2007年第6期492-493,共2页
Anhui Medical and Pharmaceutical Journal
基金
安徽省自然科学基金项目(编号:050430901)
安徽省科技厅年度重点项目(编号:05023090)
