摘要
目的 用聚合酶链反应（PCR）技术鉴定拟杆菌.方法 以该属细菌的标准16S Rrna基因序列为靶基因设计通用引物,对该属标准菌株及不同来源的临床株进行PCR扩增,然后对扩增产物进行基因测序验证引物的特异性和敏感性.结果 设计的引物能扩增13株拟杆菌,而40株肠杆菌科细菌、本实验室分离和保存的其他非拟杆菌不能被扩增;13株拟杆菌所得的PCR产物经基因测序、比对相应标准菌的16S Rrna,同源性在81%～100%.结论 建立的方法可特异的鉴定拟杆菌,敏感性为100个细菌的基因组.
Objective To identity bacteria of bacteroides group by PCR. Method The universal primers were designed according to the sequence of 16S rRNA, then 13 strains bacteroides and the other non-bacteroides were amplified by PCR. PCR products were then sequenced and analyzed by Blast. Results Thirteen strains of bacteroides were amplified, and the non-bacteroides were not amplified. The pairs of primers had highly specificity. Comparing the gene sequences of the 13 strains, bacteroides with corresponding standard 16S rRNA sequences,the homology was from 81% to 100%. Conclusion The established PCR assay could specially amplify bacteroides and had good sensitivity with 100 genomes/ml.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2007年第3期170-171,共2页
Chinese Journal of Clinical Laboratory Science
基金
河南省科技攻关项目(0523031700)。
