摘要
目的观察胃肠道间质瘤(GIST)中Nestin和C-kit在蛋白和转录水平的表达,分析Nestin在GIST诊断、鉴别诊断及发生发展中的意义。方法应用免疫组化、逆转录(RT)-PCR和Western印迹法,检测116例胃肠道间质瘤中Nestin、C-kit的表达(其中22例同时收集新鲜组织),并收集10例正常胃肠道组织和15例平滑肌瘤作为对照。结果116例胃肠道间质瘤中104例(89.7%)Nestin表达阳性;53例良性和63例恶性GIST中分别有47例(88.7%)和57例(90.5%)Nestin表达阳性;Nestin表达水平在良、恶性组间差异无统计学意义(X^2=0.1,P>0.05)。116例GIST中C-kit蛋白的阳性表达率为94.8%(110/116),Nestin和C-kit表达水平间差异无统计学意义(X^2=1.084,P>0.05)。RT-PCR和Western印迹结果显示GIST均有Nestin和C-kit表达。与正常胃肠道组织和平滑肌瘤比较,差异均有统计学意义。结论在GIST中有Nestin高表达,与C-kit联合检测有助于GIST的诊断,但不能作为判断肿瘤危险度的指标。
Objective To investigate the expression of Nestin and C-kit in gastrointestinal stromal tumors and evaluate their role in the diagnosis and pathogenesis of gastrointestinal stromal tumors （GISTs）. Methods The expressions of Nestin and C-kit in 116 cases of GISTs were evaluated by immunohistochemistry, RT-PCR and Western-Blot. The result was compared with 15 cases of leiomyomas and 10 cases of normal gastraintestinal tissues. Results Immunohistochemical analysis revealed an over and diffused expression of Nestin in 104 out of the 116 cases of GISTs（89. 7%）. The expression of Nestin was positive in 90.5 % of malignant GISTs and 88.7% of benign GISTs with no significance difference between benign and malignant GISTs （Χ^2 = 0.1, P 〉 0.05）. The C-kit was expressed in 94.8% （110/ 116） of GISTs. There was no statistical difference between the expression of Nestin and C-kit （Χ^2 = 1. 084, P〉 0.05）. The Nestin and C-kit expressions can be detected in all cases of GISTs by RT-PCR and Western Blot. Conclusion In conjunction with C-kit, Nestin may be a useful marker for diagnosis of GISTs but it cannot be used as a tumor differentiation index.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2007年第5期314-317,共4页
Chinese Journal of Digestion
关键词
胃肠道间质瘤
诊断
危险度
Gastrointestinal stromal tumors
Diagnosis
Differential
