摘要
目的探讨环氧合酶(COX)-2及肝细胞凋亡在非酒精性脂肪性肝炎(NASH)发生中的作用。方法雄性SD大鼠30只,随机分为模型组、乙醇灌胃对照组和正常对照组,每组各10只。模型组给予高脂饮食(普通饲料基础上加10%猪油、2%胆固醇和5%蛋黄粉)。乙醇灌胃对照组在普通饲料中加乙醇灌胃喂养。正常对照组给予普通饲料。动物喂养12周后处死并取肝脏组织,用免疫组化法及逆转录(RT)-PCR法检测COX-2表达,TUNEL法观察肝细胞凋亡。结果模型组(0.97±0.32)和乙醇对照组(0.89±0.19)大鼠COX-2表达水平均明显高于正常对照组(0),差异有统计学意义(P<0.01)。模型组(3.26±0.90)和乙醇对照组(3.33±0.78)的肝细胞凋亡指数明显高于正常对照组(0.80±0.65),差异亦有统计学意义(P<0.01)。模型组与乙醇对照组之间COX-2表达和肝细胞凋亡指数差异均无统计学意义(P>0.05)。结论COX-2在NASH发生中发挥着重要作用,肝细胞凋亡是NASH发生的重要机制。
Objective To investigate the role of cyclooxygenase（COX）-2 and hepatocyte apoptosis in the pathogenesis of nonalcoholic steatoheptatitis （NASH） . Methods Thirty male SD rats were divided randomly into three groups. The rats in NASH group （n= 10） were fed with high fatty diet, the rats in alcoholic steatoheptatitis （ASH） group （n= 10） were fed with standard diet with alcohol oral layage, and the rats in control group（n=10） were fed with standard diet for 12 weeks. Hepatic COX-2 expression was determined by RT-PCR and immunohistochemistry. Hepatocyte apoptosis was determined by TUNEL assay. Results The mRNA expression of COX-2 and hepatocyte apoptosis were significant higher in NASH group （0.97±0. 32 and 3. 26±0.90, respectively） and ASH group （0.89±0. 19 and 3.33±0. 78, respectively） than those in control group（0 and 0. 80±0. 65, respectively, P〈0. 01）. There was no significant difference of COX-2 expression and hepatocyte apoptosis between NASH group and ASH group （P〉0. 05） . Conclusion COX-2 may play an important role in the pathogenesis of NASH. The hepatocyte apoptosis is an important mechanism of NASH pathogenesis.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2007年第5期326-329,共4页
Chinese Journal of Digestion
基金
上海市高校青年基金(SJW0101)
关键词
脂肪肝
环氧合酶2
肝细胞
凋亡
Fatty live
Cyclooxygenase 2
Hepatocytes
Apoptosis
