摘要
铁皮石斛Dendrobium officinale是一种名贵药用植物,野生资源亟待保护,必须通过试管苗快繁技术研究,才能满足人们对它的需要。通过不同基本培养基对铁皮石斛进行种子发芽试验,不同消毒方法对茎尖进行存活率试验,不同植物生长调节物质组合对不定芽、原球茎和壮苗及根系的诱导试验的结果表明:铁皮石斛胚培养最佳培养基为1/2 MS(Murashigeand Skoog)+添加物A+25 g.L-1蔗糖;4种消毒方法中最佳消毒方法为B,外植物茎尖存活率可达76.2%。不定芽诱导最佳培养基为1/2 MS+1.5 mg.L-16-苄基腺嘌呤(BA)+0.25 mg.L-1萘乙酸(NAA),诱导倍数4.7。原球茎诱导最佳培养基为1/2 MS+1.0 mg.L-1BA+0.5mg.L-1NAA,诱导率为58%。不定芽最佳生根培养基为1/2 MS+0.5 mg.L-1NAA,生根率为100%,平均生根数为7.1条。建立了铁皮石斛试管苗快繁体系,为其广泛应用提供了依据。
Dendrobium officinale （iron-sheet dendrobium） is a rare plant species for medical use. Its present resource cannot satisfy the needs. Tissue culture is a quick and convenient path to produce more iron-sheet dendrobium. The tiny seeds were sowed on the basic culture medias of MS （Murashige and Skoog）, half of MS （ 1/2 MS）, Hyponexl and Hyponex 2. And the fresh stems were sterilized in different ways, then cultured in the basic media added NAA or BA in different ammounts. The resluts showed that 1/2 MS ＋ A （the additive substance） ＋ 25 g·L^-1 sugar was the best medium for seeding; Method B was the best disinfection with the livability 76.2% ; 1/2 MS ＋ 1.5 mg·L^-1 BA ＋ 0.25 mg·L^-1 NAA was better for adventitious buds induction, with 4.7 buds on each explant; 1/2 MS ＋ 1.0 mg·L^-1 BA ＋ 0.5 mg·L^-1 NAA was fine for protocorm induction, with the rate of 8% ; 1/2 MS ＋ 0.5 mg·L^-1 NAA was better for rooting, with a 100% rate and 7.1 roots on each explants. The proliferation systerm can enlarge the application of Dendrobium officinale. [ Ch, 1 fig. 5 tab. 11 ref. ]
出处
《浙江林学院学报》
CSCD
北大核心
2007年第3期372-376,共5页
Journal of Zhejiang Forestry College
基金
浙江省科学技术重点项目(2005C22082)
关键词
植物学
铁皮石斛
组织培养
不定芽
原球茎
botany
Dendrobium officinale
tissue culture
adventitious buds
protocom
