宫颈病变程度与人类乳头瘤病毒(16、18型)负荷的关系被引量：1

Grade of cervical disease are associoated with human papillomaviruses 16,18 type load

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摘要目的探讨宫颈病变程度与人类乳头状瘤病毒(HPV)16、18型负荷的关系。方法采用实时荧光定量聚合酶链反应(FQ-PCR)方法,检测宫颈不同病变活检组织及石蜡包埋标本中HPV16、18型感染状况。结果正常宫颈上皮感染HPV16、18型阳性率为3%,CINⅠ、Ⅱ、Ⅲ级和宫颈癌感染HPV16、18阳性率分别为45.7%,69.5%,72.2%和74.6%。正常宫颈与CIN,宫颈癌HPV16、18阳性率差异有极显著性(P<0.0001)。宫颈糜烂(Ⅰ°、Ⅱ°、Ⅲ°),CIN(Ⅰ、Ⅱ、Ⅲ级)及鳞癌HPV16、18阳性标本平均拷贝数分别为:3.58×103、1.80×105、1.19×107,随着病变程度加重而逐渐增加,差异有极显著性(P<0.0001)。结论宫颈病变程度与HPV16、18型负荷呈正相关。对宫颈病变组织标本行HPV16、18型检测,对判断宫颈病变发展趋势,积极处理癌前病变,阻断病程、预防宫颈癌的发生将起到重要作用。 Objective： The association between human papillomavirus （HPV） 16, 18 type DNA copy number and grade of cervical disease was investigated. Methods： Real time fluorescence quantitative PCR （FQ - PCR）, which combines PCR and fluorescence probe hybridization, was used to measure HPV 16, 18 DNA copy number in 606 cases specimens of different cervical disease biopsy and embeded in paraffin. Results： Normal cervical epithelium HPV16, 18 DNA was detected positive 1 case （ 1/32, 3% ）, copy number 5.0 × 10^2, erosion of cervix positive 35 cases （35/241, 14. 5% ）, average copy number 3.58 × 10^3 , cervical intraepithelial neoplasia （CIN） positive 56 cases （56/100, 56% ）, average copy number 1.80 × 10^5, squamous cell carcinoma positive 168 cases （ 168/225 74. 7% ） , average copy number 1.19 × 10^7. The cellular HPV16, 18 DNA copy number increased significantly with increasing grade of cervical disease （ P 〈 0. 0001 ）. Conclusion： Grade of cervical disease are associoated with human papillomaviruses 16, 18 type load. Detected HPV16, 18 DNA plays a very important role in diagnosis and treatment of different cervical disease.

作者谢军花蔡兰邹先进

机构地区湖北省荆门市第一人民医院病理科

出处《中国优生与遗传杂志》 2007年第6期56-57,126,共3页 Chinese Journal of Birth Health & Heredity

关键词宫颈病变人乳头状瘤病毒聚合酶链反应荧光光度测定法 Cervical disease Human papillomavirus Polymerase chain reaction Fluorophotometry

分类号 R737.33 [医药卫生—肿瘤]

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