摘要
０．５ＭＰａＰＥＧ－６０００胁迫２４ｈ明显刺激三叶期玉米幼苗，第三叶质膜ＡＴＰａｓｅ活性提高，并且Ｖｍａｘ增加近一倍，而Ｋｍ值却降低；水分胁迫下渗透调节因子脯氨酸累积，Ｋ含量则呈现先上升后下降的趋势，渗透调节的起动与质膜ＡＴＰａｓｅ活性上升可能无直接关系。实验也表明质膜ＡＴＰａｓｅ活性被三氟拉嗪（ＴＦＰ）促进而受钙调素抑制，由此推断质膜ＡＴＰａｓｅ在钙调素参与的磷酸化后活性降低。
The PM ATPase activity of the third maize leaves significantly increased in the three leaves seedlings under 0.5MPa PEG 6000 for 24 hours,and V max rised almost one time,but K m decreased.With the development of water stress,proline accumulated, treatment of vanadate, a inhibitor of ATPase ,delay proline accumulation 24 hours. Total contents rised,then declined with or without vanadate. These suggested that the start of osmoregulation may be not related with the increasing of ATPase activities. Trifluoperazine,a inhibitor of calmodulin, stimulated calmodulin and inhibited PM ATPase activities. It suggested that ATPase activities may be declined after phosphorylation.
出处
《华北农学报》
CSCD
北大核心
1997年第1期30-33,共4页
Acta Agriculturae Boreali-Sinica
基金
河北师范大学生物系
关键词
玉米
幼叶
质膜
水分胁迫
钙
渗透调节
Maize leaves
Plasma membrane
ATPase
Water stress
Calmodulin
Osmoregulation
