摘要
在传统的甘油—卵黄—柠檬酸盐稀释液的基础上，Ｂ液内分别添加了３种浓度的蔗糖、牛血清白蛋白（ＢＳＡ）、锌离子进行分析比较。结果表明，添加蔗糖的保护液较传统的保护液能更有效地冻存精液，１００ｍＭ组精子活力显著提高（Ｐ＜０．０５）。ＢＳＡ和锌离子对精液冻存无显著影响（Ｐ＞０．０５），但都具有一定的保护作用。改良稀释液的精子活力比传统稀释液的提高３２．７％（Ｐ＜０．０１）。细管精液的冻存效果显著高于颗粒精液（Ｐ＜０．０５）。用最佳组合的细管精液对１２５只母羊进行人工授精，情期受胎率为６３．１％。
In order to improve the sperm motility of the frozen—thawed goat semen,the sucrose,BSA and Zn 2+ were added into the conventional cryoprotective medium—the egg yolk—glycerol—citric acid medium The results showed that effect of cryoprotective medium supplemented with sucrose on cryoprotection of spermatozoa was better than that of traditional glycerol medium without supplement BSA and Zn +2 had little effect on sperm survival Maximum post—thawing motility was obtained when goat semen was diluted with 100mM sucrose,6% BSA and 8mM Zn 2+ cryoprotective medium 125 of cycling ewes were inseminated with mixed frozen—thawed semen and a pregnancy rate in one estrous cycle of 63 1% was obtained
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1997年第2期120-125,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
