摘要
目的观察咪达唑仑、盐酸戊乙奎醚单用或合用对小鼠学习记忆长时间的影响。方法80只KM小鼠分层随机区组设计,分为咪达唑仑(1mg/kg,midazolan,Mid)组、盐酸戊乙奎醚(0.2mg/kg,penehyclidine,Pen)组、咪达唑仑+盐酸戊乙奎醚(1mg/kgMid+0.2mg/kgPen,Mid+Pen)组及生理盐水(10ml/kg,NS)组,每组20只;每组再随机分为测试记忆获得组和巩固组,每组10只。行为学检测采用避暗实验,以训练前/后给药分别评估药物对小鼠记忆获得或巩固的影响。比较各组避暗实验训练后d1、d2、d3、d4、d5、d6和d7测验所得的潜伏期和错误次数。结果①Mid单用或与Pen合用对记忆的获得和巩固均有抑制作用并维持较长一段时间;②Mid合用Pen与Mid单用相比,不加重记忆获得的抑制,而明显加重记忆的巩固,且合用比单用对记忆抑制时间长。结论Mid和Pen在麻醉前使用,对防止术中知晓有利,但可能是POCD的一个因素。
Objective To investigate the long - term effects of midazolam and penehyclidine hydrochloride on learning and memory function of mice. Methods According stratified random block design ,80 KM mice were divided into 4 groups ： midazolam lmg/kg（ group M, n = 20 ） , penehyclidine hydrochloride 0.2mg/kg （ group P, n = 20 ） , midazolam lmg/kg ＋ penehyclidine hydrochloride 0.2mg/kg （group M ＋ P, n = 20 ） and control group （group NS, n = 20 ） ;20 mice in each group were divided randomly into testing memory acquisition （ n = 10 ） and memory consolidation（ n = 10） further. For behavioral testing a step - through passive avoidance test was used, in order to evaluate the effects of the agents administruted on the memory acquisition before fraining and on the memory consolidation immediately after fraining. The step - through latencies and the numbers of errors 1,2,3,4,5,6 and 7 day after the training were recorded. Results Administration of midazolam impaired memory acquisition and consolidation when administrated alone or in combination with penehyclidine hydrochloride, and this effect persisted for 3 days . Administration of midazolam combinated with penehyclidine hydrochloride did not worsen the effect on memory acquisition, but worsen the effect on memory consolidation obviously. Furthermore, administration of midazolam combinated with penehyclidine hydrochloride impaired memory function persisting longer than that of administration of midazolam alone. Conclusions Administration of midazolam and penehyclidine hydrochloride as premedication was advantageous for prevention of awareness during operation, nevertheless was attributed to one of the causations of POCD.
出处
《医学研究杂志》
2007年第6期38-41,共4页
Journal of Medical Research
基金
国家自然科学基金资助项目(39970715
30471657)
江苏省自然科学基金资助项目(BK2001143)
关键词
咪达唑仑
盐酸戊乙奎醚
学习记忆
Midazolam
Penehyclidine hydrochloride
Learning and memory
