摘要
目的结合既往工作,探讨肿瘤坏死因子α(TNFα)作用后大鼠肺微血管内皮细胞(RPMVEC)cAMP浓度的变化及其与β-AR变化的关系。方法在100ml培养瓶内接种等量RPMVEC,细胞单层汇合后分3组:TNFα组加5000U/mlTNFα,TNFα+山莨菪碱组加5000U/mlTNFα+10μg/ml山莨菪碱,正常对照组加等量稀释液(各组n=4)。于作用后15、90min采用放免法测定RPMVEC cAMP浓度。结果正常RPMVEC cAMP浓度为11.83±1.35fmol/μl。TNFα组和TNFα+山莨菪碱组,15min和90min两个时相点细胞内cAMP浓度均显著高于正常对照组(P<0.01)。TNFα+山莨菪碱组15min与TNFα组15min比较,细胞内cAMP浓度显著增高(P<0.01);TNFα+山莨菪碱组90min与TNFα组90min比较,胞内cAMP浓度无显著差异(P>0.05)。TNFα组15min与90min比较,胞内cAMP浓度显著增高(P<0.05)。结论TNFα作用可引起RPMVEC cAMP浓度显著升高,其机制可能与腺苷酸环化酶活化有关,而与TNFα引起的β-AR变化下调无关。
Objective To investigate the effects of tumor necrosis factorα（TNFα） on cAMP concentration in rat pulmonary microvascular endothelial cells（RPMVEC） and the relationship between cAMP and β - AR on the basis of our work in the past. Methods Equal amount of RPMVEC was incubated in 100 ml flasks,which were divided into 3 groups when the cell monolayer confluented. 5000U/ ml TNFα added in TNFα group, 5000U/ml TNFα and 10μg/ml anisodamine added in TNFα ＋ anisodamine group, equal amount of diluent added in normal control group and n =4 in each group. The cAMP concentration in each group was measured at 15 and 90min with radioimmunoassay. Results The cAMP concentration in normal RPMVEC was 11.83 ± 1.35fmol/μl. The cAMP concentration in TNFα group and TNFα ＋ anisodamine group at 15 and 90 minute were significantly higher than those in the normal control group （P 〈 0.01 ）. The cAMP concentrtion in TNFα ＋ anisodamine group was significantly increased than that in TNFα group at 15 minute（P 〈0.01 ）. There was no significant difference between the cAMP concentrations in TNFα group and TNFα ＋ anisodamine group at 90 minute （P 〉 0.05 ） ,and the cAMP concentration in TNFα group at 15 minute was increased significantly compared with 90 minute （ P 〈 0.01 ）. Conclusions TNFα can lead to the increase of cAMP concentration in RPMVEC with an unknown mechanism which may related with the activation of adenylyl cyclase and do not attribute to the down regulation of β -AR induced by TNFα.
出处
《医学研究杂志》
2007年第6期54-56,共3页
Journal of Medical Research
