摘要
建立了利用HPLC法测定奶粉中泛酸的方法:色谱柱:Zorbax SB-C18;流动相:乙腈∶0.02mol/LKH2PO4=4∶96;流速:1mL/min;进样量:20μL;DAD检测器,波长200nm。方法在1μg/mL￣20μg/mL之间线性良好,泛酸的回收率在86%￣102.87%之间,RSD值在0.44%￣3.23%之间。对市场上几种奶粉的检测发现,泛酸含量均符合要求,有的产品中泛酸含量远高于标示值。
A high performance liquid chromatographic method was adapted for the assay of pantothenic acid in milk powder.The chromatographic system included a Zorbax SB-C18 column （5 μm 4.6 mm×250 mm） and a mobile phase consisting of a sodium dihydrogen phosphate dihydrate buffer and acetonitrile （96：4,v/v）,with a flow rate of 1 mL/min.The column effluent was monitored by DAD at 200 nm.The system was linear from 1 μg/mL -20 μg/mLThe recoveries of pantothenic acid from samples ranged from 86 %-102.87 %,and the value of RSD ranged from 0.44 %-3.23 %.The result of assaying of pantothenic acid for some milk powder products showed： they were in compliance with recommendation and pantothenic acid contents of some products were higher than those declared on their nutritional labels.
出处
《食品研究与开发》
CAS
北大核心
2007年第6期121-124,共4页
Food Research and Development
关键词
高效液相色谱
泛酸
奶粉
HPLC
pantothenic acid
milk powder
