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根癌农杆菌介导的枇杷花药胚状体遗传转化中抗生素种类和浓度的研究

Research of Antibiotics Species and Concentration on Loquat Anther Embryos Transformation by Agrobacterium tumefaciens
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摘要 研究了安必西林、噻孢霉素和硫酸庆大霉素对根癌农杆菌EHA52的抑制效果以及对枇杷胚状体生长的影响。结果表明:安必西林、噻孢霉素浓度高于500 mg/L、硫酸庆大霉素浓度高于300 mg/L时,对农杆菌EHA52均有较好的抑制效果。但是,硫酸庆大霉素对枇杷胚状体的生长有很强的抑制效果。安必西林的浓度在800 mg/L时为最佳浓度。 The inhibitive effect of Ampicilin, Cefotaxime and Gentamycin on Agrobacteniuam tumefaciens EHA52 and its effect on the growth of loquat embryos. The results showed that proliferation of Agrobacterium tumefaciens was completely suppressed when the concentration of Ampicilin and Cefotaxime in the medium was higher than 500 mg/L and the concentration of Gentamycin in the medium was higher than 300 mg/L. But the growth of loquat embryos was suppressed in the medium containing Gentamycin. Medium containing 800 mg/L Ampicilin had the best effect on growth of loquat embryos.
出处 《安徽农业科学》 CAS 北大核心 2007年第16期4723-4723,4745,共2页 Journal of Anhui Agricultural Sciences
关键词 抗生素 抑制作用 枇杷 根癌农杆菌 花药胚状体 遗传转化 Antibiotics Agrobacterium tumefaciens Inhibition Loquat
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