摘要
目的:建立测定草乌甲素多囊脂质体包封率的方法。方法:采用离心法分离草乌甲素多囊脂质体,HPLC测定总药量和游离草乌甲素量并计算样品包封率。结果:低速离心时,转速不影响包封率的测定;3批样品的平均包封率为87.12%。结论:离心法操作简单、准确,可用于测定草乌甲素多囊脂质体的包封率。
AIM： To establish a method of determining the entrapment efficiency of bulleyaconitine A multivesicular liposomes. METHODS： Bulleyaconitine muhivesicular liposomes and its free bulleyaconitine were separated by centrifugalization. To calculate the entrapment efficiency, total and free bulleyaconitine were assayed by HPLC. RESULTS： At low speed, the rate of rotation did not affect the determination of entrapment efficiency. The average entrapment efficiency of three lot samples was 87.12%. CONCLUSION： Entrapment efficiency of bulleyaconitine muhivesicular liposomes could be evaluated simply and quickly by centrifugalization.
出处
《中成药》
CAS
CSCD
北大核心
2007年第6期825-827,共3页
Chinese Traditional Patent Medicine
关键词
草乌甲素
多囊脂质体
包封率
离心
bulleyaconitine A
multivesicular liposomes
entrapment efficiency
centrifugalization
