摘要
目的:建立3种冲洗剂细菌内毒素的检查方法。方法:按照2005年版《中国药典》(二部)附录ⅠS和ⅪE进行试验及结果判定。结果:呋喃西林灭菌溶液对鲎试验的凝集反应无干扰作用;硫酸新霉素灭菌溶液在pH值为3.9～5.8时无干扰作用,pH值为3.2～3.8时可以通过4倍稀释消除干扰;醋酸氯己定灭菌溶液的干扰作用用稀释法难以消除。结论:细菌内毒素检查法中的凝胶法可控制呋喃西林灭菌溶液和硫酸新霉素灭菌溶液的细菌内毒素限度。
OBJECTIVE： To establish a method of bacterial endotoxins test for monitoring pyrogen in three sterile irrigations. METHODS： The experiment was performed, and the results were assessed in accordance with Appendixes Ⅰ S and Ⅺ E, Volume Two, Chinese Pharmacopoeia 2005 Edition. RESULTS： The tests showed that there was no interference with the agglutination reactions between Tachypleus Amebocyte Lysate（TAL） and endotoxins in furacillin sterilized solution and neomycin sulfate sterilized solution （pH3.9-5.8） . The inhibition of neomycin sulfate sterilized solution with pH value 3.2-3.8 can be removed by fourfold dilution. But the inhibitory action of chlorhexidine acetate sterilized solution was hard to be eliminated. CONCLUSION： The method can control the limit of bacterial endotoxins in furacillin and neomycin sulfate sterilized solution.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第16期1262-1264,共3页
China Pharmacy
关键词
呋喃西林灭菌溶液
硫酸新霉素灭菌溶液
醋酸氯己定灭菌溶液
细菌内毒素检查法
Irrigation
Furacillin sterilized solution
Neomycin sulfate sterilized solution
Chlorhexidine acetate sterilized solution
Bacterial endotoxins test
