摘要
应用ELISA、LAT、IHA血清试验对8头人工感染弓形虫猪的血清抗体滴度变化规律进行了监测,并对不同检测方法的结果进行了比较。结果发现,试验猪在皮下感染弓形虫RH株速殖子后,血清中的特异性抗体首次检出的时间方面,LAT(第4d)早于ELISA(第14d)和IHA(第20d);检出持续时间方面,LAT(54d)长于ELISA(44d)和IHA(19d);平均检出率方面,LAT(7/8)高于ELISA(6.7/8)和IHA(5/8)。试验结果表明,ELISA和LAT适合于猪弓形虫病的诊断和血清学调查,LAT更适合于猪弓形虫病的早期检测。
Observation and comparison of the dynamic changes of specific antibody were done by detecting antibody titer in sera of swines inoculated hypodermically the tachyzoites of Toxoplasma gondii RH strain by ELISA, LAT and IHA. The special antibodies in the sera of the infected swine were detected primarily on the day 14th by ELISA, 4th by LAT and 20th IHA post-infection. The detected continual time of LAT and ELISA was 54 days and 44 days respectively, longer than that of IHA （19 days） and the average detected rate of them was also higher than that of IHA. The results showed that ELISA and LAT were suitable for investigating serology and diagnosis of swine toxoplasmosis, and the latter was more suitable for diagnosis of toxoplasmosis in initial stage of the infection.
出处
《湖北农业科学》
北大核心
2007年第3期427-430,共4页
Hubei Agricultural Sciences
基金
湖北省科技攻关计划项目(2006AA205A02)
