摘要
1日龄艾维茵肉鸡健雏420只,随机分为7组,分别喂以对照日粮(Cu 11 mg/kg)和高铜日粮(Cu 100mg/kg,高铜Ⅰ组;Cu 200 mg/kg,高铜Ⅱ组;Cu 300 mg/kg,高铜Ⅲ组;Cu 400 mg/kg,高铜Ⅳ组;Cu 500 mg/kg,高铜Ⅴ组;Cu 600 mg/kg,高铜Ⅵ组)6周,以流式细胞术和透射电镜的方法观察研究了高铜对雏鸡免疫器官淋巴细胞凋亡的影响。电镜下观察,与对照组比较,高铜Ⅳ、Ⅴ和Ⅵ组免疫器官淋巴细胞发生凋亡的频率明显增高。发生凋亡的淋巴细胞表现为染色质凝聚、边集,细胞核呈新月形、“C”形、花环状(或圆斑状)及花瓣状。流式细胞术测定显示,高铜Ⅳ、Ⅴ和Ⅵ组免疫器官淋巴细胞凋亡百分率也明显高于对照组和高铜Ⅰ、Ⅱ、Ⅲ组。上述研究结果表明,饲粮铜含量超过300 mg/kg可以诱导淋巴细胞凋亡。
The experiment was conducted with the objectives of examining the effect of dietary high copper on lymphocyte apoptosis of the lymphoid organs in the chicken by the methods of ultrastructural pathology and Flow Cytometry （FCM）. 420 one-day-old Avian chickens were randomly divided into seven groups, and fed on diets as follows：control （Cu 11 mg/kg）, and high copper （Cu 100 mg/kg, high copper group Ⅰ ; Cu 200 mg/kg, high copper group Ⅱ ; Cu 300 mg/kg,high copper group Ⅲ ;Cu 400 mg/kg,high copper group Ⅳ ;Cu 500 mg/kg, high copper group Ⅴ ;Cu 600 mg/kg,high copper group Ⅵ）for six weeks. Uhrastructurally, the frequency of lymphocyte apoptosis was higher in high copper groups Ⅳ, Ⅴ and Ⅵ than in control group and high copper groups Ⅰ, Ⅱ, Ⅲ. The mitochondria of lymphocytes of lymphoid organs were swelled, and its crista were broken or/and disappeared. The apoptotic lymphocytes showed that the chromatin become condensed and marginated, and the shape of nuclei were crescent, C- shaped, dark round and petal-like. Also, the statistical analyses by FCM indicated that the percentage of apoptotic cells of the lymphoid organs was higher in high copper groups Ⅳ, Ⅴ and Ⅵ than in control group and high copper groups Ⅰ, Ⅱ, Ⅲ. The aforementioned results demonstrated that dietary copper in excess of 300 mg/kg diet could induce apoptosis in lymphocytes of lymphoid organs.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2007年第6期601-607,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金资助项目(30471304)
四川省教育厅重点项目(2002A002)
科技厅重点项目(03JY029-030-2)
关键词
高铜
淋巴细胞凋亡
超微结构
流式细胞术
雏鸡
dietary high copper
lymphocyte apoptosis
ultrastructural pathology
flow cytome try
chicken
