摘要
目的:介绍目前实验室应用的几种抗真菌药物联用的体外药效评价方法。方法:通过查阅文献,综述各种方法的具体操作及优缺点。结果:多种方法可评价抗真菌药物联用的体外药效,如棋盘法、时间-杀菌曲线法、E-test法、双纸片扩散法等。结论:用于评价抗真菌药联用效果的各方法均有其优点及不足之处,且有良好的一致性,目前实验室中最为普遍应用的方法是棋盘法。
Objective： To present a review of methods commonly applied to assess the pharmacodyna- mics in vitro of the combination therapy of antifungal drugs. Methods： To introduce the operation procedures, advantages and disadvantages of the methods via consulting references. Results： The effect of different antifungal drugs in combination can be determined using the checkerboard method, time-kill curve method, E- test and double disk diffusion method. Conclusions： The methods to evaluate the effect of antifungal drugs in combination have both advantages and disadvantages and have a good agreement with each other. The checkerboard method was most frequently used in laboratory at the time.
出处
《抗感染药学》
2007年第2期51-55,共5页
Anti-infection Pharmacy
基金
山东省科技攻关课题(编号:2005GG4402036)
关键词
抗真菌药物
棋盘法
时间-杀菌曲线
体外药效
Antifungal drugs
checkerboard
time-kill curve
pharmacodynamics in vitro
