摘要
对热研2号柱花草无菌实生苗的胚轴与叶片切段进行愈伤组织培养研究。结果表明，MS＋NAA2mg／L＋BA0．5mg／L及MS＋2，4-D3mg／L＋BA0．3～0．5mg／L＋CH200mg／L对叶片切段诱导效果最好。而下胚轴愈伤组织的诱导以MS＋NAA3mg／L＋BA0．1～0．3mg／L和MS＋2，4-D3mg／L＋BA0．5mg／L最优。愈伤组织在MS＋BA2．5mg／L＋NAA0．1mg／L进行分化培养，可实现高频植株再生。最适生根培养基为1／2MS＋1．0mg／LIBA＋0．5mg／LIAA，生根率可达95．84％。
Studies on callus culture and plant regeneration of Stylosanthes guianensis cv. Reyan 2 showed that the optimum callus induction media were MS＋NAA 2 mg/L＋BA 0.5 mg/L and MS＋2,4-D 3 mg/L＋BA 0.3 -0.5 mg/L＋CH 200 mg/L for leaf pieces, and MS＋NAA 3 mg/L＋BA 0. 1-0. 3 mg/L and MS＋2,4-D 3 mg/L＋BA 0. 5 mg/L for hypocotyl segments from aseptic seedlings. High-frequency bud differentiation and plant regeneration was achieved on MS＋BA 2. 5 mg/L＋NAA 0. 1 mg/L. The best rooting medium was 1/2 MS ＋1.0 mg/L IBA＋0.5 mg/L IAA.
出处
《草业学报》
CSCD
2007年第3期136-138,共3页
Acta Prataculturae Sinica
关键词
热研2号柱花草
组织培养
愈伤组织
植株再生
Stylosanthes guianensis cv. Reyan2
tissue culture
callus
plant regeneration
