摘要
分别利用肉汁胨琼脂、营养琼脂、胰酶大豆琼脂、马铃薯葡萄糖琼脂、脑心浸液琼脂、金氏培养基B、LB营养琼脂和酵母膏蛋白胨琼脂8种常用的细菌分离培养基来分离小麦内生细菌,以选择适宜的分离培养基,结果显示,胰酶大豆琼脂和金氏培养基B的分离效果显著优于其他培养基,马铃薯葡萄糖琼脂和脑心浸液琼脂的分离效果较差。利用选择出的金氏培养基B和胰酶大豆琼脂分别对豫东麦区的7个小麦品种的内生细菌进行分离,进一步确认了胰酶大豆琼脂和金氏培养基B为分离小麦内生细菌的适宜培养基。
Seven isolating media, including Beef extract Peptone Agar （BPA）, Nutrient Agar （NA）, Tryptic Soy Agar （TSA）, Potato Dextrose Agar （PDA）, Brain Heart Infusion （BHI）, Kings medium B（KMB）,Luria-Bertani medium（LB） and Yeast extract Peptone Agar（YPA）, were utilized to isolate endophytic bacteria from wheat, aiming at screening the optimal media. The results showed that the isolation effect of TSA and KMB was better than that of other media, while the PDA and BHI media had the lowest isolation effect. The two media TSA and KMB were further confirmed to be the optimal media by using eight wheat varieties from eastern part of Henan province to isolate endophytic bacteria.
出处
《河南农业科学》
CSCD
北大核心
2007年第5期63-65,70,共4页
Journal of Henan Agricultural Sciences
基金
河南省教育厅自然科学基金(200510475036)
河南大学重点基金(04ZDZR005)
关键词
内生细菌
小麦
培养基
分离
Endophytic bacteria
Wheat（Triticum aestivum）
Medium
Isolation
