期刊文献+

血浆(1→3)-β-D-葡聚糖与抗深部白色念珠菌感染药物疗效关系的实验研究 被引量:29

The relation between plasma(1→3)-β-D-glucan and the therapeutic effect on systemic candidasis in mice
下载PDF
导出
摘要 目的探讨深部真菌感染小鼠血浆(1→3)-β-D-葡聚糖水平与抗真菌药物疗效的关系。方法110只ICR小鼠随机分成3组,空白对照组、模型组、氟康唑组,建立深部白色念珠菌感染的动物模型。应用MB-80微生物动态快速检测系统及GKT-5MSet动态真菌检测试剂盒定量检测血浆中(1→3)-β-D-葡聚糖的含量,双肾组织匀浆液培养计数菌落数。结果①造模后24小时模型组菌落计数与血浆(1→3)-β-D-葡聚糖的水平比空白对照组明显升高,差异具有显著性(P<0.05)造模成功。②氟康唑组菌落计数与血浆(1→3)-β-D-葡聚糖水平随着治疗逐渐减少,与模型组比较差异具有统计学意义(P<0.05)。③模型组各时间点及氟康唑组治疗前、治疗第1、3、7天菌落计数与血浆(1→3)-β-D-葡聚糖水平具有线性相关关系(P<0.05)。结论检测血浆(1→3)-β-D-葡聚糖是一种实用的深部真菌感染早期快速的诊断方法,(1→3)-β-D-葡聚糖的水平与抗真菌药物疗效呈线性相关,可以借助连续监测血浆(1→3)-β-D-葡聚糖的水平评估药物疗效。 Objective This study was done to evaluate the relationship between the level of plasma( 1→3 ) - β - D - glucan and the therapeutic effect on systemic candidiasis in mice. Methods One hundred and ten ICR mice were divided into three groups at random: normal control group ( 10 ) ,model group (50), and fluconazole group (50). Mice were infected by injection of Candida albicans into the lateral tail vein except norreal control group. Treatment was begun 24h post - challenge. Every 10 mice of the model group and fluconazole group were randomly sacrificed at pretherapy, and the 1 st day,3 rd day,5th day and 7th day of treatment. Content of plasma( 1→3 ) - β -D -glucan were measured by Microbiology Kinetic Rapid Reader MB - 80. Two kidneys were aseptically removed and homogenized in 5 ml of sterile saline. The plates were incubated for 48 h at 37℃ before quantitating Candida growth by colony counts. Results ①The kidney colony count and plasma( 1→3 ) - β - D - glucan of the model group were heightened 24h after injection compared to those of the normal control group. The differences were significant( p 〈 0.05 ). It indicated that the model established successfully and the increased abnormally of plasma( 1→3 ) -β -D -glucan could reflect the existence of sys- temic candidiasis. ②The kidney colony count and plasma( 1→3 ) - β - D - glucan of the fluconazole group were decreased gradually along with treatment. The differences were significant between fluconazole group and model group in kidney colony count and plasma( 1→3 ) - β -D -glucan level( P 〈0.05). ③The kidney colony count and plasma( 1→3 ) -β -D- glucan level of each time point of the model group and those of the pretherapy ,1 st day, 3rd day,Tth day of the fluconazole group shown a perfect linear correlation ( P 〈 0.05 ). Conclusion Plasma( 1→3 ) - β - D - glucan measurement is a practicable method in early diagnosis of systemic candidiasis, continuous monitoring plasma ( 1→3 ) - β - D - glucan is useful for the evaluation of therapeutic effect of antimicrobial medicine.
出处 《临床和实验医学杂志》 2007年第6期20-22,共3页 Journal of Clinical and Experimental Medicine
基金 北京市中医药管理局重点学科项目资助
关键词 (1→3)-β—D-葡聚糖深部白色念珠菌感染抗真菌治疗 ( 1→3 ) - β - D - glucan Systemic candidiasis Antifungal therapy
  • 相关文献

参考文献2

二级参考文献23

  • 1Yoshida M, Roth RI, Grunfeld C, et al. Soluble ( 1 - > 3)-beta-D-glucan purified from Candida albicans: biologic effects and distribution in blood and organs in rabbits. J Lab Clin Med, 1996, 128:103.
  • 2Eisen DP, MacGinley R, Christensson B, et al. Candida tropicalis vertebral osteomyelitis complicating epidural catheterisation with disease paralleled by elevated D-arabinitol/L-arabinitol ratios. Eur J Clin Microbiol Infect Dis, 2000, 19:61 - 63.
  • 3Wong B, Bernard EM, Gold JW, et al. The arabinitol appearance rate in laboratory animals and humans: estimation from the arabinitol/creatine ratio and relevance to the diagnosis of candidiasis. J Infect Dis, 1982, 146:353-359.
  • 4Maertens J, Verhaegen J, Demuynck H, et al. Autopsy-controlled prospective evaluation of serial screening for circulating galactomannan by a sandwich enzyme-linked immunosorbent assay for hematological patients at risk for invasive Aspergillosis. J Clin M
  • 5Becker MJ, de Marie S, Willemse D, et al. Quantitative galactomannan detection is superior to PCR in diagnosing and monitoring invasive pulmonary aspergillosis in an experimental rat model. J Clin Microbiol,2000, 38:1434 - 1438.
  • 6Aketagawa J, Tanaka S, Tamura H, et al. Activation of limulus coagulation factor G by several ( 1 - > 3 ) -beta-D-glucans: comparison of the potency of glucans with identical degree of polymerization but different conformations. J Biochem ( Tokyo), 1993,
  • 7Denning DW. Early diagnosis of invasive aspergillosis. Lancet, 2000,355:423 - 424.
  • 8Christensson B, Sigmundsdottir G, Larsson L. D-arabinitol-a marker for invasive candidiasis. Med Mycol, 1999, 37:391 -396.
  • 9Yeo SF, Zhang Y, Schafer D, et al. A rapid, automated enzymatic fluorometric assay for determination of D-arabinitol in serum. J Clin Microbiol, 2000, 38:1439 - 1443.
  • 10Walsh TJ, Merz WG, Lee JW, et al. Diagnosis and therapeutic monitoring of invasive candidiasis by rapid enzymatic detection of serum Darabinitol. Am J Med, 1995, 99:164 - 172.

共引文献27

同被引文献194

引证文献29

二级引证文献110

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部