摘要
本文报道热空气、大肠杆菌K_（12）D_（31）诱导柞蚕蛹血淋巴,提高溶菌酶活力的方法。应用选择性热变性、等电点沉淀、DEAE—纤维素离子交换层析、CC—纤维素亲和层析等分离技术从柞蚕蛹血淋巴中提取溶菌酶,溶菌酶比活力达36700μ/mg蛋白,提高了150倍,活力回收达48.6％。
Induced by hot atmosphere and E. coli (K12D31), lysozyme extraction from immuned haemolymph was studied. Lysozyme was separated from pupae by following steps, heat denaturation, isoelectric precipitation, DEAE-cellulose ion-exchanged column chromatography and chitin-coated cellulose affinify chromatography the relative activity of the lysozyme preparation was 36700 units/mg protein, which was 150 times of the control. The total activity recovery rate was 48.6%.
出处
《工业微生物》
CAS
CSCD
1997年第1期21-24,共4页
Industrial Microbiology
关键词
柞蚕蛹
溶菌酶
分离
纯化
Chinese oak silkworm pupae, Lysozyme, Isolation, Purification.
