摘要
采用盆栽实验,研究了在模拟酸雨、镍单一或复合污染的红壤中,莴笋的生长和叶片中抗氧化酶活性、活性氧和丙二醛含量的变化。结果表明,单一酸雨污染或酸雨与25 mg/kg镍的复合污染对植株生长无明显影响;酸雨增强了50-100 mg/kg镍对莴笋生长的抑制效应。复合污染下,酸雨为pH4.5时,莴笋叶片超氧化物歧化酶（SOD）、过氧化氢酶（CAT）的总体活性较单一镍污染高,而酸雨为pH3.5时则两者活性降低。复合污染下,叶片过氧化物酶（POD）活性升高,抗坏血酸过氧化物酶（APX）活性降低,且随酸雨pH降低,变化的幅度增大;同时,O2^＊ˉ的产生加快,H2O2和MDA含量升高,表明叶片组织遭受了氧化胁迫和膜脂过氧化损伤,这可能是复合污染下莴笋生长受阻的重要原因。
Pot experiments were conducted to measure the growth and the changes in activities of different antioxidant enzymes, the levels of active oxygen species and malondialdehyde （MDA） in lettuce plants （Lactuca sativa var. angustana Irish） in red soil contaminated with simulated acid rain and nickel, applied alone or in combination. The results showed that the acid rain alone, or applied in combination with 25 mg/kg nickel did not significantly affect plant biomass, while the acid rain enhanced the inhibition of plant growth when 50- 100 mg/kg nickel was applied to the soil. Compared with those of single nickel pollution, the general activities of superoxide dismutase （SOD） and catalase （CAT） in leaves were increased by acid rain of pH 4.5 sprayed in combination with nickel, but decreased when the acidity of acid rain reached pH 3.5. The combined pollution increased the activity of peroxidase （POD）, but decreased the activity of ascorbate peroxidase （APX） with acid rain intensity-dependent manner, i. e. , the lower acidity of acid rain, the greater changes in the enzyme activities. Simultaneously, the generating rate of O^＊ˉ and the content of H2O2 and MDA were more higher than those of nickel alone, suggesting oxidative damage and membrane lipid peroxidation occurred in leaves, and it might be the important reason for the growth inhibition of plants subjected to the combined pollution.
出处
《水土保持学报》
CSCD
北大核心
2007年第3期99-102,共4页
Journal of Soil and Water Conservation
基金
国家"863"计划资助项目(2005AA219040)
关键词
镍
模拟酸雨
复合污染
抗氧化酶
莴笋
红壤
nickel
simulated acid rain
combined pollution
antioxidant enzyme
lettuce
red soil
