摘要
在碱性介质中,铜氨络离子的催化作用下,石吊兰素对乙腈-H_2O_2化学发光体系有强烈的增敏作用.在Hypersil ODS色谱柱上,以乙腈-N,N-二甲基甲酰胺-0.01mol/L草酸(体积比27:6:67)为流动相的条件下,实现了对石吊兰素的分离测定,建立了测定石吊兰素的方法.发光强度与石吊兰素的浓度在0.02～50mg/mL范围内呈线性关系.检出限(3σ)为0.01μg/mL.5次平行测定的相对标准偏差分别为1.24%～1.56%.应用于吊石苣苔中石吊兰素的测定,结果满意.
An HPLC-CL linkage method was based on the strong sensitive chemiluminescence of the CH3CN-H2O2 and Cu（NH3）4 I]^2＋ system in alkaline medium. The separation was carried out on a Hypersil ODS column with a mobile phase of CH3CN：HCON2 （CH3）2 ： 0. 01mol/LH2C2O4 （27 ： 6 ： 67, V/V/V）. The linear ranges for Nevadensin determination was 0. 02～50μg/mL with a detection limit（3σ） of 0.01μg/mL. The relative standard deviation for tetracycline were from 1.24% to 1.56% （n= 5）. The method can be useful for the determination of Nevadensin in Lysionotus Pauciflorus Maxim samples.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第3期48-51,共4页
Journal of Southwest China Normal University(Natural Science Edition)
