摘要
目的：建立以反相同效液相色谱法测定毛鱼藤中鱼藤酮含量的方法。方法：以ZORBAX Eclipse XDB—C18（5μm，4.6×150mm）色谱柱为固定相；乙腈：0.1％磷酸溶液（48：52，v／v，pH2.6）为流动相；流速1．0mL／min；柱温40．0℃；检测波长299nm。结果：鱼藤酮在浓度为30～50mg／L范围内，其峰面积与浓度线性关系良好，线性方程为A=20．7311C-0．3616（r=0.9998），平均加样回收率为100．74％，RSD为0．86％。结论：本方法操作简便、快速．结果准确．可用于毛鱼藤中鱼藤酮的含量测定。
Objective：To establish a reversed-phase high performance liquid chromatography method for rotenone in Derris elliptica, Methods ：The determination was performed on ZORBAX Eclipse XDB-C18 （5 μm,4.6 × 150 mm）column. The mobile phase consisted of acetonitrile and 0.1% phosphate acid solution （48 ：52, v/v, pH 2.6） with flow rate at 1.0 mL/min and determination wavelength at 299 nm under 40.0 %, Results ： Under the conditions described, the calibration curve of rotenone was linear over the concentration range of 30 to 50 mg/L. The regression equation was ： A = 20.731 1C-0.361 6, r = 0. 999 8 （ n = 5 ,P 〈 0.01 ）. The average recovery for rotenone was 100.74%. The mean relative standard deviation （RSD） was 0.86%. Conclusion：The method is simple and accurate, and suitable for the determination of rotenone in Derris elliptica.
出处
《药学实践杂志》
CAS
2007年第3期159-161,共3页
Journal of Pharmaceutical Practice
关键词
毛鱼藤
鱼藤酮
反相高效液相色谱法
Derris elliptica
rotenone
reversed-phase high performance liquid chromatography
