摘要
目的检测HER-2和K-RAS在结直肠癌中的表达情况,探讨两者在结直肠癌形成中的作用、相互关系以及用于预后评价的价值。方法运用免疫组织化学法检测12例正常结直肠黏膜组织、15例结直肠腺瘤、123例结直肠癌及25例淋巴结转移灶中HER-2和K-RAS的表达情况。结果HER-2和K-RAS的高表达率在结直肠癌分别为67.5%和51.2%,在结直肠腺瘤分别为80.0%和33.3%,而在正常结直肠黏膜组织则分别为33.3%和0。HER-2和K-RAS在结直肠癌和结直肠腺瘤中的高表达率均显著高于正常结直肠黏膜组织(P<0.05),但结直肠癌和结直肠腺瘤之间比较差异均无显著性。在结直肠癌中,HER-2和K-RAS的联合高表达率为36.3%。胞膜HER-2高表达率为38.2%,与浸润深度相关。K-RAS的高表达与临床病理指标无相关性。在淋巴结转移灶中,HER-2和K-RAS的高表达呈正相关(r=0.458,P<0.05)。HER-2和K-RAS的高表达与预后均无显著相关性(P>0.05)。结论HER-2和K-RAS可能与结直肠癌的发生有关,在结直肠癌的发展过程中可能起协同作用。HER-2胞膜的高表达与结直肠癌的侵袭性有关。HER-2和K-RAS的高表达与预后均无相关性。
Objective To explore the relationship among HER-2 gene, K-RAS gene, the carcinogenesis mechanism of colorectal cancer and the prognosis by the determination of the expression of HER-2 and K-RAS in colorectal cancer tissues. Methods The expression of HER-2 and K-RAS was detected with immunohistochemical methods in normal mucosal tissue, colorectal adenoma, colorectal cancer and metastatic lymph nodes. Results The positive rates of HER-2 and K-RAS high expression were 67. 5 % and 51.2 %, respectively, in colorectal cancer, and 80. 0% and 33.3 % respectively in colorectal adenoma, while those in normal mucosal tissue were only 33. 3% and 0. The expression of HER-2 and K-RAS in colorectal cancer and colorectal adenoma were significantly higher than those in normal mucosal tissue, but no significant differences existed between colorectal cancer and colorectal adenoma. In colorectal cancer, high expression of both proteins occurred in 36.3 % of the cases. The positive rate of HER-2 hyperexpression in cell membrane was 38. 2 %, which showed a positive correlation with depth of invasion, high expression of K-RAS was not correlated with clinicopathologic parameters. In the metastatic lymph nodes, the HER-2 hyperexpression was positively correlated with K-RAS hyperexpression （r=0. 458, P〈0. 05）. There was no significant association between their high expressions and prognosis in colorectal carcinoma. Conclusion HER-2 and K-RAS may play a combined role in the development of colorectal cancer. Hyperexpression of HER-2 in cell membrane was significantly associated with invasiveness. There was no significant association between their high expressions and prognosis in colorectal carcinoma.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2007年第6期598-600,共3页
Medical Journal of Chinese People's Liberation Army
