摘要
为了解长春市慢性乙型肝炎患者血清中的乙型肝炎病毒(HBV)基因型情况及其与临床特点的相关性,应用型特异性引物进行巢式PCR方法对长春市69例慢性乙型肝炎患者血清HBV进行基因分型检测。在69例血清标本中,B型10例(占14.5%);C型41例(占59.4%);B+C混合型8例(占11.6%);未分型的患者共10例(占14.5%)。C基因型患者的HBV-DNA定量、HBeAg阳性率明显高于B基因型患者(HBV-DNA:P<0.01;HBeAg:χ2=3.98,P<0.05),C基因型患者肝功检查指标谷丙转氨酶(ALT)和总胆红素(TB IL)均较B基因型患者高(P<0.01)。长春地区存在HBV B基因型、C基因型、B+C混合基因型及未分型,C基因型为优势基因,引起的肝脏活动性炎症较B基因型明显。
In order to study the situation genotypes of sera in hepatitis B virus （HBV） and reciprocity of their clinical characteristics of ehronie hepatitis B （HB） patients in Changehun city, HBV genotypes were determined by nested- PCR analysis using type-specific primers from randomly selected HB positive patients. The results showed that of 69 patients, 10 （14.5%） were genotype B, 41 （59.4%） were genotype C, 8 （11.6%） were mixed genotype B ＋C, and 10 （ 14.5% ） were unknown genotype. HBV-DNA and the positive rate of HBeAg of genotype C patients are higher than those in genotype B patients （ HBV-DNA ： P 〈0.01 ; HBeAg： χ2 = 3.98, P 〈0.05） ATL and TBIL of genotype C patients are higher than those in genotype B patients （P 〈0.01 ）. Therefore, genotype B, genotype C, genotype B ＋ C, and unknown genotype exist in Changehun area, and genotype C is the dominant genotype in this area and caused more evident avidity of liver inflammation than those of genotype B.
出处
《微生物学杂志》
CAS
CSCD
2007年第3期27-29,共3页
Journal of Microbiology
