摘要
目的探讨槐耳清膏体外逆转人肝癌细胞多药耐药性的作用及可能的机制。方法MTT法检测槐耳清膏的细胞毒作用和处理前后耐药细胞对化疗药物的敏感性;流式细胞仪检测细胞内阿霉素浓度;RT-RCR检测MDR1基因的表达。结果槐耳清膏在0.01g/L以下剂量时对HepG2和HepG2/ADM耐药细胞株的抑制率均<10%,半数抑制率(IC_(50))分别为0.917 g/L和1.66 g/L,无细胞毒剂量即0.008 g/L的槐耳清膏能部分逆转HepG2/ADM细胞的耐药性,与之合用使耐药肝癌细胞对阿霉素、顺铂、丝裂霉素、氟尿嘧啶的相对逆转效率分别为79%、73.5%、97.4%和86.7%,HepG2/ADM细胞内阿霉素浓度明显增加,MDR1表达下降了33.7%。结论槐耳清膏具有体外逆转人肝癌细胞多药耐药性的作用,可能与下调MDR1表达、增加细胞内化疗药物积累有关。
Objective To investigate the MDR-reversing effect of Extraetum trametes robiniophila murr on human hepatoeellular carcinoma HepG2/ADM in vitro and its potential mechanism. Methods MTT was used to test the toxicity of the Chinese medicine of Extraetum trametes robiniophila murr and the ehemosensitivity to ehemotherapeuties in Extraetum trametes robiniophila murrtreated HepG2 and HepG2/ADM cell lines. Flow eytometry was used to determine intraeellular ADM （Adriamyein） concentration. The expression of MDR1 was measured by RT PCR. Results The inhibition rate of HepG2 and HepG2/ADM caused by Extractum trametes robiniophila murr was less than 10% under the dose of 0.01 g/L, and the IC50 were 0. 917 g/L and 1.66 g/L. Extraetum trametes robiniophila murr（0. 008 g/L） partly overcame the MDR of cell line HepG2/ADM and decreased the drug resistance. The relative reverse effieieneies to ADM, eisplatin （eDDP） , mytomyein （MMC） , 5 fluororacil （5-FU） were 79%, 73.5%, 97.4% and 86.7%, respectively. After exposure to 0. 008 g/ L Extractum trametes robiniophila murr, the concentration of ADM in HepG2/ADM was significantly increased and MDR1 expression in HepG2/ADM cells decreased by 33.7%. Conclusion Extractum trametes robiniophila murr can reverse the multi-drug resistance of human hepatoeellular carcinoma in vitro. The possible mechanism is related to down-regulating the expression of MDR1 and increasing the concentration of drug inside the cells.
出处
《中华肝胆外科杂志》
CAS
CSCD
2007年第6期385-388,共4页
Chinese Journal of Hepatobiliary Surgery
关键词
癌
肝细胞
多药耐药
槐耳清膏
Carcinoma,hepatoeellular
Multidrug resistance, Chinese medicine Extraetum trametes robiniophila murr
