摘要
目的研究盐附子(SAC)对小鼠神经系统的急性毒性作用。方法采用小鼠自发活动、爬杆、Morris水迷宫试验,分别设SAC生药7.68、3.84、1.92 g.kg-1剂量组,ig给药,同时设阴性对照和阳性对照组。单次给药后15、60 min用自发活动仪测定小鼠在10 min内自发活动的次数;单次给药30 min后将小鼠放在爬杆架的玻璃棒上,记录小鼠爬杆时间;连续给药4 d,末次给药后30 min进行水迷宫试验,以小鼠找到隐藏平台的时间为逃避潜伏期。结果SAC高、中、低剂量组的小鼠自发活动次数、爬杆时间、逃避潜伏期与对照组相应指标相比,差异无统计学意义(P>0.05)。结论SAC浓度为7.68、3.84、1.92 g.kg-1时,未观察到其对小鼠神经系统的急性毒性作用。
OBJECTIVE To evaluate the acute neurobehavioral toxicity of Salted aconite root（SAR） on mice by assay of spontaneous activity, grasping pole, and Morris water maze. METHEODS The mice were administered by garages, with raw SAR of 7.68,3.84, 1.92 g·kg^-1. The spontaneous activity frequency of 15 and 60 minutes after dosing was observed. The mice were put on the glass pole 30 minutes after single exposure to SAR. The grasping pole time was observed. After 4 days continuous administration, the time that mice found the hiding platform as escape latency time after the last dosing was observed. RESULTS The frequency of spontaneous activity, grasping pole time and escape latency time had no significant differences between Salted aconite root dosing groups and the negative control group （P〉0.05）. CONCLUSION The raw Salted aconite root with 7.68,3.84,1.92 g·kg^-1 had no acute neurobehavioral toxicity on mice.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2007年第3期300-301,共2页
West China Journal of Pharmaceutical Sciences
基金
国家自然科学基金资助项目(批准号:30230410)
关键词
盐附子
自发活动次数
爬杆时间
逃避潜伏期
神经毒性
Salted aconite root
Assay of spontaneous activity
Assay of pole gasping
Assay of Morris water maze
Neurobehavioral toxicity
