摘要
目的建立测定温经通络酒中龙胆苦苷含量的方法。方法采用HPLC法,Kromasil C18色谱柱(150 mm×4.6 mm,5μm);流动相为甲醇-水(1:4);流速1.0 ml.min-1;检测波长254 nm。结果龙胆苦苷在0.24-4.8μg范围内线性良好(r=0.9999);平均加样回收率为100.9%,RSD=2.7%;日内日间精密度分别为0.2%、1.6%。结论所建方法简便、灵敏、准确,适合于温经通络酒中龙胆苦苷的含量测定。
OBJECTIVE To develop an HPLC method for the determination of the gentiopicroside in Wenjing Tongluo Jiu. METHODS The separation was performed on a Kromasil C18 column（ 150 mm×4.6 mm,5μm）with the mobile phase composed of methanol - water（ 1 ： 4） at flow rate of 1.0 ml· min^-1. The ultraviolet detector was operated at 254 nm. RESULTS A good linearity was obtained in the range of 0.24-4.80 μg（ r =0. 9999）. The average recovery was 100.9% with RSD of 2. 7% , the within -day and the between - day RSD were 0.2% and 1.6%, respectively. CONCLUSION The method is simple, rapid and applicable to the determination of the gentiopicroside in Wenjing Tongluo Jiu.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2007年第2期206-207,共2页
West China Journal of Pharmaceutical Sciences
关键词
温经通络酒
秦艽
龙胆苦苷
高效液相色谱法
Wenjing Tongluo Jiu
Radix gentianae macrophyllae
Gentiopicroside
HPLC
