摘要
目的探讨白细胞介素-6(IL-6)基因多态性与尘肺易感性的关系。方法采用1∶1病例对照研究方法,以经市尘肺病诊断组确诊的尘肺患者为病例,对照严格按年龄、民族、接尘性质、接尘剂量进行匹配。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和PCR方法检测IL-65个位点的基因型和等位基因频率。结果病例对照共120对,两组的吸烟率差异无统计学意义(P>0.05)。分析发现,-174位点和-597位点仅检出GG基因型。-572位点基因型及等位基因频率在病例与对照的分布差异无统计学意义(P>0.05)。-634位点CC基因型病例组高于对照组,差异有统计学意义(P<0.05)。C等位基因分布频率在病例组和对照组分别为77.5%和69.6%,差异有统计学意义(P<0.05)。VNTR位点仅发现等位基因D和E,仅检测DD和DE基因型,该位点等位基因和基因型在病例组及对照组的分布差异无统计学意义(P>0.05)。结论该研究未发现IL-6(-174、-597)位点存在基因多态性。未发现IL-6(-572)位点、VN-TR位点基因多态性与尘肺易感性有关。研究提示携带IL-6(-634)CC基因型的接尘者更易患尘肺。
Objective To explore the relationship between genetic polymorphism of interleukin-6 and pneumoconiosis. Methods 120 male stage Ⅰ pneumoconiosis were selected as the eases who were examined with radiography and diagnosed by the Pneumoconiosis Diagnosis Expert Panel, based on the Chinese National Diagnosis Criteria of Pneurnoconiosis （GBZ70-2002）. 120 dust exposure workers were selected as control who had been exposed to the same dust and matched with the cases by 1： 1. The differences of age and cumulative length of service between case and control were not over five years and two years, respectively. 5ml peripheral venous blood was drawn from each subject on the spot and was anticoagulated with 2% EDTA. The polymorphisms of IL-6 （ -174, -572, -597, -634 and VNTR loci） were detected with the method of polymerase chain reactionrestriction fragment length polymorphism （PCR-RFLP） techniques and PCR. Results The differences of age, the length of exposure to dust, cumulative length of service, smoking frequencies between the cases and the controls were not significant （P 〉 0. 05 ）. IL-6 （-174） genotype GG and （-597） genotype GG were barely defected. There were no significant difference between pneumoconiosis and the controls （P 〉 0. 05） on distribution of genotype and allele for IL-6 （ - 572）. The frequencies of IL-6 （ - 634） allele had statistical difference between the cases （77.5%） and the controls （69.6%）. D allele and E allele of IL-6 （VNTR） were only found among all subjects, and the frequencies of D allele were 93.3% and 95.0% in the case and the control groups and the difference was not significant （P 〉0. 05）. Conclusion Gene polymorphism in IL-6 （ - 174） and （-597） were not found. The relationship between genetic polymorphism of IL-6 （-572） and pneumoconiosis was not observed. D and E alleles of IL-6 （VNTR） were found among studied subjects but no significant difference existed between the cases and the controls. Workers carrying IL-6 （-634） genotype CC were likely to increase the risk of pneumoconiosis.
出处
《中国职业医学》
CAS
北大核心
2007年第3期199-201,204,共4页
China Occupational Medicine
关键词
尘肺
IL-6
基因多态性
易感性
Pneumoconiosis
Interleukin-6
Gene polymorphism
Susceptibility
