摘要
目的检测荷瘤鼠体内S-100蛋白及RANTES(T细胞特异性趋化因子)的表达,探讨其与肿瘤发生和转移的关系。方法将培养的B16黑色素瘤细胞于实验组C57小鼠背部皮下注射,待肿瘤形成后,取局部淋巴结和肿瘤病变中心区及交界区皮肤,作免疫组化染色。结果肿瘤早期组S-100蛋白和RANTES阳性反应物的面密度和数密度在淋巴结和肿瘤组织、癌周皮肤明显高于正常对照组和肿瘤晚期组(P<0.05)。结论S-100蛋白和RANTES的表达程度与肿瘤的侵袭和进程呈负相关。
Objective To study the relationship of the development and metastasis of B16 melanoma with expressions of S-100 protein and RANTES（regulated on activation, normal T-ceU expressed and secreted）. Methods Cultured B16 melanoma cells were injected into the back of C57 mice, skin of the surrounding and center tissues of the tumor and the regional lymph nodes were obtained after forming tumor and examined by immunohistoehemistry staining. Results The area density and number density of S-100 protein and RANTES positive product in lymph nodes and tumor tissue and surrounding skins were much higher in earlier stage of the tumor than in later stage of the tumor and in the normal control（P〈0.05）. Conclusion The relationship of the expressions of S-100 protein and RANTES with the invasion and progress of B16 melanoma was negative.
出处
《解剖科学进展》
CAS
2007年第2期138-140,共3页
Progress of Anatomical Sciences
基金
黑龙江省自然科学基金资助项目(No.D0234)
