摘要
目的观察青春期前SD鼠精索血管高位离断后对青春发育前期、青春发育期、性成熟期睾丸形态结构的影响。方法日龄为30 d(青春发育前期)SD大鼠麻醉后,分别行左侧腹腔内精索血管离断术(手术组)、左侧腹腔内精索血管游离术(对照组),术后将每大组大鼠再分成3组(手术组每组12只;对照组每组5只),分别在术后d3(手术后急性期)、d30(青春发育期)、d56(性发育成熟期)获取睾丸进行组织形态学研究。结果青春期前SD鼠精索血管高位离断后,手术侧睾丸在急性期(d3)出现缺血受损改变;青春发育期(d30)出现萎缩改变;性发育成熟期(d56)发生完全萎缩,附睾管内精子消失。结论青春期前SD鼠精索血管高位离断术可引起急性期手术侧睾丸缺血,影响青春发育期手术侧睾丸发育。该手术引起性发育成熟期后手术侧睾丸完全萎缩,生精功能丧失。
Objective To investigate the effects of high spermatic vessel dissection on testiculax morphological alteration of SD rats in prepubetty, puberty and sexual maturity phases. Methods Thirty - day - old SD rats were divided into 2 groups underwent sham operation and left high spermatic vessel dissection as a simulation of Palomo＇s maneuver. Detailed morphological investigations were made at 3 different postoperative intervals among the 3^rdd day,30^th day and 56^th day. Results High spermatic vessel dissection in prepubertal rats induced acute testiculax ischemia in the operated testes on the 3^rd day. Most of the operated testes on the 30^th day showed testiculax atrophy. And all the operated testes showed testiculax atrophy and sperm disappearance in epididymis on the 56^th day. Conclusion High dissection of spermatic vessel in prepubertal rats induced testiculax ischemia in prepuberty and testicular growth failure in puberty,testiculax atrophy completely and sperm production losing in sexual maturity phase.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第11期817-819,共3页
Journal of Applied Clinical Pediatrics
基金
温州市科技计划基金项目资助(Y2005A111)
