摘要
目的探讨白术黄芪汤(DAA)益气健脾作用的物质基础。方法DAA以原方配伍水提,煮沸浓缩至相当于药材1500g.L-1;DAA单药提取部位组方(PEAAG)以组成DAA的3个单药白术、黄芪和甘草提取部位白术糖复合物(AMPS-Ⅱ)、黄芪总皂苷(TSA)和甘草总黄酮(TFG)以4∶3∶3比例混合,加蒸馏水混匀研磨配成储存液,含提取物100g.L-1。用三硝基苯磺酸(TNBS)制备大鼠溃疡性结肠炎模型,造模d2分别灌胃给予DAA(相当于药材15g.kg-1)和PEAAG(含提取部位0.3g.kg-1)连续15d,肉眼观察大鼠结肠病变并进行组织病理学检查。用TNBS制备小鼠结肠炎模型,于d2分别灌胃给予PEAAG及其3个组成部位(剂量均为0.2g.kg-1)连续10d,观察结肠组织髓过氧化物酶(MPO)活性的变化。用一次最大耐受量实验观察DAA和PEAAG的小鼠急性毒性。结果与模型对照组比较,PEAAG组结肠的肉眼和组织病理学评分均降低,DAA组变化不明显。PEAAG和AMPS-Ⅱ可降低TNBS致小鼠结肠炎结肠组织MPO的活性,PEAAG作用更明显,TSA和TFG作用不明显。DAA和PEAAG一次最大耐受量分别为60g.kg-1和4g.kg-1,相当于临床用量的180倍和300～600倍。结论单药提取部位组方PEAAG对溃疡性结肠炎具有较好的治疗作用,在所观察的剂量条件下可能优于原方DAA和3个提取部位,且毒性未增加。
AIM To investigate the material base of invigorating qi and spleen of the decoction of Atractylodes macrocephala Koidz. and Astragalus mongholicus Bge. （DAA）, composed of Atractylodes, Astragalus and Glycyrrhiza in weight ratios of 10： 7： 3. METHODS DAA was decocted with water and concentrated to 1500 g· L^-1（ corresponding to raw-materials）. The prescription of extracts from Atractylodes, Astragalus and Glycyrrhiza （PEAAG） was composed of the complex polysaccharides from Atractylodes macrocephala （AMPS-Ⅱ ）, total saponin of Astragalus （TSA） and total flavonoids of Glycyrrhiza （TFG） in 4： 3： 3. PEAAG was dissolved in water to make the concentration 100 g· L^-1. The ulcerative colitis rat and mouse models were made by infusion of 50 and 125 mg· L^-1 2,4,6-trinitrobenzene sulfonic acid（TNBS） , respectively, into colon on d 1. On d 2 DAA 15 g·kg^-1 and PEAAG 0.3 g· kg^-1 were given （ig） to the rats of DAA and PEAAG groups, respectively, for 15 d. The macroscopical and histopathological changes of rat colons were observed and the scores were counted on d 17. PEAAG and its 3 component drugs （ all 0.2 g· kg ^-1 ） were given （ig） to the mice, respectively, for 10 d. The myeloperoxidase （MPO） activity of mouse colon tissue was detected. Experiments of acute toxicity of DAA and PEAAG in mice were carried out to test their maximal tolerated doses. RESULTS Compared with the model control group, the macroscopical and histopathological scores of rats in PEAAG group decreased, and no significant decreases in DAA group. PEAAG and AMPS- Ⅱ decreased the MPO activity of the colon tissue of ulcerative colitis mice, the effect of PEAAG was stronger. TSA and TFG had no effect on MPO activity. The maximal tolerated dose of DAA and PEAAG was 60 g· kg^- 1 and 4 g· kg^-1, respectively , which corresponded to as much as 180 times and 300 -600 times of clinical doses, PEAAG, the respectively. CONCLUSION prescription composed of fractions extracted from Atractylodes, Astragalus and Glycyrrhiza, has therapeutic effect on ulcerative colitis and has no evident toxicity.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2007年第3期223-228,共6页
Chinese Journal of Pharmacology and Toxicology
基金
国家自然科学基金项目(90209004)
广东省自然科学基金项目(05102323)
上海市教育委员会E-研究院建设计划项目(E03008)~~
关键词
白术黄芪汤
组方
提取部位
治疗
结肠炎
溃疡性
急性毒性试验
decoction of Atractylodes and Astragalus
prescription, extracts
therapy
colitis, ulcerative
acute toxicity tests
