摘要
目的:探讨凋亡抑制蛋白(inhibitor of apoptosis protein,IAP)家族的新成员Livinα和Livinβ及其基因在人口腔鳞癌组织中的表达及意义。方法:采用反转录聚合酶链反应法(RT-PCR)检测20例口腔鳞癌组织、15例口腔软组织囊肿和10例癌旁正常口腔黏膜组织中LivinαmRNA和LivinβmRNA的表达,并用Western印迹检测2种Livin蛋白在3种组织中的表达情况,应用SPSS10.0软件包对结果进行统计学分析。结果:19例口腔鳞癌组织中均有Livinα和LivinβmRNA的表达,14例口腔软组织囊肿和10例癌旁正常口腔黏膜组织中均可见Livinα或LivinβmRNA的表达,3者表达率无统计学差异,但Livinα和LivinβmRNA在口腔鳞癌组织中的表达显著高于口腔软组织囊肿和癌旁正常口腔黏膜组织(P<0.01),Western印迹检测结果和RT-PCR结果一致。结论:Livin可能在人口腔鳞癌的发生、发展中起着重要作用。
PURPOSE： To investigate the expression of the newly found inhibitor of apoptosis protein Livinα and Livinβ and their gene expression in human oral squamous cell carcinoma. METHODS： Twenty specimens of human squamous cell carcinoma（SCC）, 15 cases of benign cysts, 10 cases of normal oral mucosal tissues adjacent to the cancerous lesions were examined. Polymerase chain reaction with reverse transcription （RT-PCR） and Western blot analysed were used to detect mRNA and protein expression of Livin, respectively.The results were analysed by SPSS10.0 software package. RUSULTS： Livin protein and mRNA expression was detected in 19 SCC tissues,14 benign cysts and 10 normal oral mucosal tissues adjacent to the cancerous lesions.But the expressions were significantly higher in oral SCC than in benign cysts and normal oral mucosal tissues （P〈0.01）. CONCLUSION： Livin may play an important role in the tumorigenesis and development of human oral squamous cell carcinoma. Supported by National Natural Science Foundation of China （Grant No.30672331）.
出处
《上海口腔医学》
CAS
CSCD
2007年第3期315-318,共4页
Shanghai Journal of Stomatology
基金
国家自然科学基金(30672331)
关键词
LIVIN
基因
口腔
鳞癌
Livin
Gene expression
Oral
Squamous cell carcinoma
