摘要
目的探讨巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)对新生微血管生成及血管内皮生长因子(VEGF)、Smadl 基因表达的影响。方法以体外培养的人血管内皮细胞株 EA.hy926为研究对象,分别用体外血管生成分析试剂盒及免疫组织化学染色法观察 MIF 在体内外对新生微血管生成的作用。通过基因芯片实验检测 MIF 对内皮细胞血管生成相关基因表达的影响,并通过 RT-PCR 检测 MIF 对 VEGF_(165)、Smadl 基因表达的影响。结果 MIF 可以促进人血管内皮细胞在体外形成血管腔,在小鼠体内也能促进新生微血管生成。MIF 可以显著上调血管内皮细胞血管生成基因21个,其中上调 VEGF_(165)、Smadl 基因表达呈剂量依赖性。结论MIF 有促进新生微血管生成的作用,并能促进人血管内皮细胞中 VEGF_(165)、Smadl 基因表达。
Objective To investigate the roles of macrophage migration inhibitory factor（MIF） in neovascularization in vitro and in vivo and the roles of MIF in VEGF165 , mothers against DPP homolog l（smadl） genes expression in endothelial cells. Methods Angiogenesis assay in vitro by MIF, a numerical value was associated with a degree of angiogenesis progression. Angiogenesis assay in vivo by MIF, the factorⅧ immunohistochemical staining was used to assay the micro vessels density. Angiogenesis gene array by using the super array gene chip. Semiquantitative detecting the gene expression of VEGF and smadl by RT-PCR. Results MIF stimulated the tube formation of endothelial cells in vitro, MIF promoted the blood vessels formation in matrigel plug in vivo. MIF up-regulated the mRNA expression of 21 angiogenesis genes,and up-regulate the mRNA expression of the VEGF and smadl in dosedependent way from 10 ng/ml to 50 ng/ml concentration. Conclusions MIF could promote neovascularization and up-regulate the mRNA expressions of VEGF, smadl in dose-dependent way.
出处
《中国心血管杂志》
2007年第3期169-171,共3页
Chinese Journal of Cardiovascular Medicine
基金
广东省自然科学基金团队项目(No.015015)
