摘要
目的:建立广西民间草药岩黄连药材中有害元素砷、汞的分析检测方法,并制订该药材中砷和汞的限量标准。方法:参照中国药典(2005年版),对样品进行消解后,采用双道氢化物发生原子荧光光谱法同时测定岩黄连药材中有害元素砷、汞的含量。结果:该方法测得砷在0～6.40 mg/L范围内线性关系良好,回归方程Y=32.72X-0.77,相关系数r=0.9997,检出限为0.02 mg/L;测得汞在0～18.00 mg/L范围内线性关系良好,回归方程Y=58.24X+2.76,相关系数r=0.9999,检出限为0.005 mg/L。广西东兰县岩黄连规范化种植基地的岩黄连药材中砷、汞的含量符合商务部颁布的《药用植物及制剂外经贸绿色行业标准》(WM/T2-2004)中重金属的限量要求。结论:该方法操作简便、快速,为岩黄连药材质量标准体系中有害成分的限量规定提供参考,并有利于促进广西东兰道地药材规范化种植过程中生产出符合无公害标准的绿色中药材。
Objective： To establish a method for quantitative determination of arsenic and mercury in Corydalis saxicola bunting by using intermittent - flow - hydride generation - atomic fluorescence spectrometry （ HG - AFS ）. Methods： According to the method described in Pharmacopoeia of the People＇s Republic of China （2005 Edition）, samples were digested in a mixture of concentrated nitric acid and perchloric acid in quartz vessels, and then were subjected to the analyses on HG - AFS. After optimizing the experimental conditions affecting the fluorescence intensity of arsenic and mercury, the quantitative analyses of arsenic and mercury in the cultivated C. saxicola were carried out by HG - AES using external calibration for quantification. Results：The detection limits of the method were 0. 02 mg/L for arsenic and 0. 005 mg/L for mercury, respectively. The levels of the contaminants of arsenic and mercury in cultivated C. saxicola bunting were below the toxic heavy metal limitations proposed by Green Standards of Medicinal Plants and Preparations for Foreign Trade and Economy. Conclusion：This method is simple and suitable for quantitative determination of arsenic and mercury in C. saxicola bunting and could be used for the safe assurance of the medicinal plant.
出处
《中国卫生检验杂志》
CAS
2007年第6期970-971,976,共3页
Chinese Journal of Health Laboratory Technology
基金
广西壮族自治区农业厅与东兰县联合资助课题(2004-2007)
关键词
岩黄连
砷
汞
氢化物发生
原子荧光光谱法
规范化种植
Corydalis saxicola bunting
Arsenic
Mercury
Hydride generation
Atomic fluorescence spectrometry
Good agricultural practice
