摘要
目的建立消炎抗菌片中没食子酸的高效液相色谱含量测定方法。方法采用Aglient ZORBAXSB-C18色谱柱（4.6 mm×250 mm，5μm）;流动相：甲醇-0.025 mol.L^-1磷酸水溶液（10∶90）;流速：0.6 mL.min^-1;柱温：室温;检测波长：215 nm。结果没食子酸在1.168-23.36μg.mL^-1范围内呈良好线性关系（r=0.999 5），平均加样回收率为99.44%，RSD为1.10%。结论本方法简便、快速、准确、重复性好，用于测定消炎抗菌片中没食子酸的含量是可行的。
OBJECTIVE To establish a HPLC method for determining the gallic acid content in the Xiaoyankangjun tablets. METHODS Aglient ZORBAXSB-Cls column （4.6 mm × 250 mm, 5 μm） was used, the mobile phase consisted of methanol-0. 025 mol· L^-1 phosphoric aqueous solution （ 10： 90） ; the flow rate was 0.6 mL· min^-1 and column temperature was set at room temperature; the detecting wavelength was set at 215 nm. RESULTS The method was linear with the range of 1.168 - 23.36 μg·mL^-1 for gallic acid （r = 0.999 5） ; the average recovery was 99.44% ; the RSD was 1.10%. CONCLUSION The method is simple, rapid and accurate. It can be used for quantitative determination of gallic acid in the Xiaoyankangjun tablets.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2007年第3期225-227,共3页
Chinese Journal of Modern Applied Pharmacy
