摘要
目的:观察苦参碱对ox-LDL诱导的单核细胞源性泡沫细胞内胆固醇脂和PPAR-γ,小凹蛋白-1表达的影响.方法:用荧光分光光度法测定胆固醇浓度;采用TBARS法检测细胞内脂质过氧化产物;用油红O染色法检测泡沫细胞的形成;用荧光RT-PCR和Western Blot检测PPARγ,caveolin-1的表达.结果:单核细胞经PMA及oxLDL共同孵育后,细胞内形成大量脂滴,胆固醇酯(CE)和细胞内脂质过氧化产物(MDA)含量由(3.4±0.6)mg/L,(0.43±0.07)nmol/L升至(64.8±6.8)mg/L,(8.50±1.23)nmol/L(P<0.05),泡沫细胞由(4.7±2.2)%升至(77.8±7.0)%(P<0.05),而PPAR-γ mRNA和PPAR-γ,小凹蛋白-1蛋白表达由(4.4±0.8),(0.5±0.09),(0.6±0.09)降至(1.6±0.4),(0.33±0.09),(0.33±0.09)(P<0.05),苦参碱低、中、高干预组细胞内积聚的CE和MDA含量分别为(29.7±4.9)mg/L,(1.92±0.46)nmol/L,(5.8±1.3)mg/L,(0.6±0.08)nmol/L,(3.4±0.6)mg/L,(0.43±0.07)nmol/L;泡沫细胞为(46.2±5.8)%,(16.3±3.4)%,(4.8±1.5)%,PPAR-γ mRNA和PPAR-γ,小凹蛋白-1蛋白表达为(6.4±2.2),(0.6±0.08),(0.5±0.09),(7.4±2.2),(0.6±0.08),(0.6±0.07),(8.6±2.7),(0.6±0.06),(0.7±0.06),与ox-LDL+PMA组比较有显著性差异(P<0.05).结论:苦参碱减少ox-LDL诱导的单核细胞源性泡沫细胞的形成及细胞内胆固醇聚集,增加泡沫细胞内PPAR-γ,小凹蛋白-1的表达.
AIM： To study the effect of matrine on PPAR-γ and caveolin-1 expressions and cholesterol ester （CE） in foam cells derived from monocytes. METHODS： The accumulation of cholesterol in monocytes was measured by fluorescence spectrophotometric method. The lipid peroxide within cells was detected by TBARS method; the foam cells were observed by oil red 0 staining. PPAR-γ mRNA and PPAR-γ, caveolin-1 levels were determined by RT-PCR and Western blot respectively. RESULTS： In the monocytes incubated with PMA and oxy-low density lipoprotein （ox-LDL）, CE and lipid peroxide （MDA） increased from （3.4 ±0.6） mg/L, and （0.43 ±0.07） nmol/L to （64.8±6.8） mg/L and （8.50±1.23） nmol/L （P〈0.05） respectively, and foam cells increased from （4. 7 ± 2. 2 ）% to （77.8 ± 7.0） % （P 〈 0.05 ）. In low, middle, high-dose matrine groups the contents of CE and MDA were （ 29.7 ± 4.9 ） mg/L and （1.92±0.46） nmol/L, （5.8 ±1.3） mg/L and （0.6± 0.08） nmol/L, （3.4±0.6） mg/L and （0.43 ±0.07） nmol/L respectively ; and the foam cells accounted for （46.2 ± 5.8 ） %, （16.3 ±3.4）%, and （4.8 ± 1.5）% respectively, the levels of PPAR-γ mRNA and PPAR-γ, caveolin-1 protein were （6.4 ± 2.2）, （0.6±0.08）, （0.5±0.09）, （7.4±2.2）, （0.6± 0.08）, （0.6±0.07）, （8.6±2.7）, （0.6±0.06）, （0.7± 0.06） respectively. The above changes were alleviated in the matrine treatment groups and the differences were significant between the matrine treatment and the ox-LDL ＋ PMA groups （ P 〈0.05 ）. CONCLUSION： The mechanism of anti-artherosclerosis by matrine may be related to its roles in reducing cholesterol accumulation and increasing the expression level of PPAR-γ and caveolin-1 in foam cells derived from monocytes.
出处
《第四军医大学学报》
CAS
北大核心
2007年第13期1164-1167,共4页
Journal of the Fourth Military Medical University
基金
宁夏自然科学基金(NZ0534)
