摘要
目的建立鼻窦炎糖浆中盐酸麻黄碱的含量测定方法。方法采用高效液相色谱(HPLC)法,色谱柱为C18柱(250mm×4.6m m,5μm),以乙腈-水-磷酸-三乙胺(3∶97∶0.1∶0.1)为流动相,检测波长为205nm。结果盐酸麻黄碱浓度进样量在0.01204～0.2408μg范围内与峰面积积分值呈良好线性关系,r=1.0000,平均回收率为98.86%,(RSD=1.14%,n=9)。结论该方法灵敏、准确、稳定,专属性强,适用于该制剂的质量控制。
Objective To establish a HPLC method for determination of ephedrin hydrochloride in Bidouyan Syrups. Methods The HPLC method was used with the phenomenex C18（250 mm×4.6 mm, 5 μm） column. The mobile phase was acetonitrile-water-phosphotic acid - triethanolamine（3 ： 97 ： 0. 1 ： 0. 1 ）, and the detection wavelength was at 205 nm. Results The calibration curve was linear（ r = 1. 000 0） in the range of 0.0120 4-0. 240 8 μg for ephedrin hydrochloride, and the average recovery rate was 98.86% （RSD = 1. 14%, n =9）. Conclusion The method is proved to be sensitive, accurate, stable and specific. It is suitable for the quality control of Bidouya Syrups.
出处
《中国药业》
CAS
2007年第14期44-45,共2页
China Pharmaceuticals
