摘要
目的研究不同浓度的染料木黄酮(genistein,GEN)对体外培养大鼠成骨细胞C-fos、碱性磷酸酶(alkaline phosphatase,ALP)及骨钙蛋白(osteocalcin)基因表达的影响。方法采用新生大鼠颅骨分离培养成骨细胞,加入不同浓度的染料木黄酮(10-8mol·L-1、10-7mol·L-1、10-6mol·L-1、10-5mol·L-1),以妊马雌酮(商品名:倍美力)(10-8mol·L-1、10-7mol·L-1、10-6mol·L-1、10-5mol·L-1)作为阳性对照组,提取总RNA,RT-PCR半定量分析其表达。结果染料木黄酮对成骨细胞C-fos、ALP及骨钙蛋白基因水平的影响呈正相关性,并与其剂量成正相关性,但这些作用均低于雌激素水平(P<0.05)。结论染料木黄酮能诱导成骨细胞C-fos、ALP及骨钙蛋白基因的表达,并与其浓度有关,作用与雌激素相似。
Objective To study the effect of different concentrations of genistein on gene expression of C-fos, ALP and osteocalcin in cultured rat osteoblasts. Methods Newborn rat calvarid osteoblastic cell were isolated and cultured. They were cultured in medium with various concentrations genistein（ 10^-8 mol·L^-1 , 10^-7 mol· L^-1 , 10^- 6 mol· L^-1, 10^-5 mol· L^-1 ） and estrogen group（ 10 ^-8 mol· L^-1 , 10^-7 mol·L^-1 , 10^-6 mol·L^-1 , 10^-5 mol· L^-1 ）. The total RNA was extracted by TRIZOL and analyzed with semi-quantitative RT-PCR. Results Genistein can increase expression of C-fos, ALP and osteocalcin in cultured rat osteoblasts. The correlation of genistein of different concentration with C-fos, ALP and osteocalcin in cultured rat osteoblasts were positive. But, the effect was weaker than these of estrogen group. （ P 〈 0.05 ）. Conclusion The genistein could increase expression of C- fos, ALP and osteocalcin. The effect was similar to estrogen but weaker than that of estrogen.
出处
《中国骨质疏松杂志》
CAS
CSCD
2007年第5期352-355,共4页
Chinese Journal of Osteoporosis
基金
北京市优秀人才基金(2003年)
