摘要
研究了衍生化试剂BSTFA对烟草中糖类的衍生化反应,并用GC—MS进行分析.烟草中的糖类用水超声提取,用C18SPE柱预分离,衍生化后用GC—MS分析测定.结果表明,该方法的检出限范围是4.7~7.6ng,加标回收率为96%~106%,相对标准偏差(RSD)为4.63%~6.06%.实验证明该方法具有较高的准确度、快速和简便的特点.
The study researched the derivative way of carbohydrates with BSTFA by GC - MS. We used the way to analysis carbohydrates of tobacco. The carbohydrates of tobacco are extracted by the ultrasonic and beforehand separated by C18 SPE . The carbohydrates are derivatived by BSTFA and then determined by gas chromatogra- phymass spectrometry. The limits of detection of this method ranged from 4.7 - 7.6 ng. The recoveries of the method ranged from 96% - 106% and the relative standard deviations of peak areas ranged from 4.63% - 6.06%. Therefore, the results prove the way is precise,quick and convenient.
出处
《云南民族大学学报(自然科学版)》
CAS
2007年第3期239-242,共4页
Journal of Yunnan Minzu University:Natural Sciences Edition