摘要
采用标准水生生物毒性实验法,将蝌蚪暴露于不同浓度的Cu^(2+)、Cd^(2+)及其混合溶液中7d,检测蝌蚪DNA的损伤,以探讨Cu^(2+)、Cd^(2+)及其复合污染对中华大蟾蜍蝌蚪DNA的影响。结果表明,Cu^(2+)、Cd^(2+)对蟾蜍蝌蚪DNA的损伤随浓度增大而加重.在0.0298mg·L^(-1)Cu^(2+)和0.855mg·L^(-1)Cd^(2+)处理组,蟾蜍蝌蚪DNA发生I型损伤;随Cu^(2+)、Cd^(2+)浓度的升高,损伤加剧,出现Ⅱ型、Ⅲ型损伤,且一定浓度的Cd^(2+)在复合污染体系中能增强Cu2+的毒性。这两种重金属对两栖动物均具有遗传毒性作用。
Amphibian populations appear to be declining around the world, and one influencing factor may be pollution from heavy metals. Heavy metals such as copper （Cu^2＋） or cadmium （Cd^2＋） have been one of the most fundamental causes of soil and water pollution in industrial and developing countries. Using Bufo gargarizans tadpoles as test subject, the impact of Cu^2＋, Cd^2＋ single and combined pollution on DNA damage was explored. The tadpoles were exposed to different concentrations of Cu^2＋ （0.029 8, 0.037, 0.049, 0.075 mg·L^-1） or Cd^2＋ （ 0.855, 1.14, 1.71, 3.42 mg·L^-1） and their interaction for 7 days, by adopting standard determination technique of aquatic biological toxicity. The results showed that DNA damage caused by Cu^2＋ and Cd^2＋ became more serious with their increasing concentration. Single-strand break was caused by 0.029 8 mg·L^-1 Cu^2＋ and 0.855 mg·L^-1 Cd^2＋, but with the Cu^2＋ and Cd^2＋ concentration increased, the double-strand break occurred. Cd^2＋ in certain concentration could increase the toxicity of Cu^2＋ in complex pollution system. These results indicated that these two heavy metals resulted in DNA damage of amphibian.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2007年第4期1361-1364,共4页
Journal of Agro-Environment Science
基金
浙江省自然科学基金资助项目(302056)
